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Original Research Article | OPEN ACCESS

Evaluation of apoptotic and growth inhibitory activity of phloretin in BGC823 gastric cancer cell

Mingqian Lu1,2,3, Qingzhi Kong1,4 , Xinhua Xu2,3, Hongda Lu4, Zhongxin Lu2,3, Wei Yu2,3, Bin Zuo2,3, Jin Su2,3, Rong Guo2,3

1Hubei University of Chinese Medicine, Wuhan 430061; 2Institute of Oncology, China Three Gorges University; 3Oncology Department, Yichang Central Hospital,Yichang 443000; 4Wuhan Central Hospital & Wuhan Institute of Oncology, Wuhan 430061, China.

For correspondence:-  Qingzhi Kong   Email: lumqyc@sina.cn   Tel:+86-0717-6486745

Received: 19 June 2014        Revised: 8 December 2014        Published: 30 January 2015

Citation: Lu M, Kong Q, Xu X, Lu H, Lu Z, Yu W, et al. Evaluation of apoptotic and growth inhibitory activity of phloretin in BGC823 gastric cancer cell. Trop J Pharm Res 2015; 14(1):27-31 doi: 10.4314/tjpr.v14i1.5

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose:To evaluate the in vitro anti-proliferative activity and probable mechanism of phloretin in human gastric cancer BGC823 cell lines.
Methods:Phloretin was isolated from apple tree leaves and identified by 1H-Nuclear Magnetic Resonance (NMR), 13C-NMR and electrospray ionization tandem mass spectrometry (ESI-MS). The inhibitory activity of the compound in BGC823 gastric cancer cells was determined by Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and soft agar colony formation assay while its apoptotic activity was detected by fluorescene staining and Western blotting analysis.
Results:The results show that the inhibitory activity of phloretin in BGC823 gastric cancer cells was mediated by induction of apoptosis and down-regulation of Bcl2 (B-cell lymphoma 2) expression. The anti-proliferative effects of phloretin was dose-dependent and inhibited the growth of BGC823 gastric cancer cells by 73 % at 30 μM; this effectively induced cleavage of anti-poly (ADP-ribose) polymerase (PARP) as well as downregulation of Bcl2 protein expression in BGC823 cells after 24 h treatment.
Conclusion:Phloretin is a promising preventive and therapeutic agent for gastric cancer.

Keywords: Phloretin, Gastric cancer, Apoptosis, ^6;-Cell lymphoma, Anti-poly (ADP-ribose) polymerase

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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