Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of the diuretic potentials of naringenin in hypercholesterolemic rats

Mohd N Ansari¹ , Majid A Ganaie¹, Tajdar H Khan², Abdul Samad², Hassan A Madkhali¹, Syed Rizwan Ahamad³

¹Department of Pharmacology; ²Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj; ³Central Laboratory, Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

For correspondence:- Mohd Ansari Email: m.ansari@psau.edu.sa Tel:+966115886037

Accepted: 20 January 2018 Published: 28 February 2018

Citation: Ansari MN, Ganaie MA, Khan TH, Samad A, Madkhali HA, Ahamad SR. Evaluation of the diuretic potentials of naringenin in hypercholesterolemic rats. Trop J Pharm Res 2018; 17(2):239-244 doi: 10.4314/tjpr.v17i2.7

© 2018 The authors.
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Abstract

Purpose: To investigate the diuretic potentials of naringenin (NGN) in obesity induced in rats by high fat diet (HFD).

Methods: To prepare HFD, normal pellet diet was crushed and thoroughly mixed with cholesterol powder (1 % w/w). The mixture was mixed with some water and made into pellets which were then oven-baked to dry. Four groups of male Wistar albino rats (n = 6) were used for the study. Normal control (group I) received normal pellet diet. Group 2 (HFD-only) was fed HFD for 28 days, while Groups 3 and 4 were co-administered HFD and NGN at doses of 50 and 100 mg/kg, respectively. All treatments were given orally, and lasted for 28 days. Twenty-four hours after the last dose of NGN, blood was collected from all rats and total cholesterol levels determined to confirm obesity. Thereafter, the rats were placed in metabolic cages and urine samples were collected at two time-points (5 and 24 h) for measurement of urine volume, urinary pH, conductivity and electrolyte levels (Na, K and Cl).

Results: Treatment with HFD resulted in significantly (p < 0.05) increased serum cholesterol level (178.83 ± 5.43 mg/dL) when compared to normal control rats (88.17 ± 4.04 mg/dL). It also led to decrease in urinary volume (~50 %) at both time points (5 and 24 h) and in excretion of urinary electrolytes (sodium, potassium and chloride ions). However, the changes in these parameters were significantly reversed by NGN administration (p < 0.05).

Conclusion: These results demonstrate the diuretic activity of NGN in HFD-induced obese rats. Thus, NGN can be further explored for use in combination with hypolipidemic agents to tackle obesity

Keywords: High-fat diet, Hypercholesterolemia, Naringenin, Obesity