Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

FAM172A inhibits breast cancer cell growth and metastasis by upregulating Notch 3

Chongyin Tang, Guozhu Wang

Department of Surgery of Thyroid and Breast, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China;

For correspondence:- Guozhu Wang Email: wgz0401_dr@126.com Tel:+862585453096

Accepted: 12 June 2022 Published: 31 July 2022

Citation: Tang C, Wang G. FAM172A inhibits breast cancer cell growth and metastasis by upregulating Notch 3. Trop J Pharm Res 2022; 21(7):1425-1430 doi: 10.4314/tjpr.v21i7.10

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the role of family with sequence similarity 172 member A (FAM172A) in breast cancer.

Methods: FAM172A expression was evaluated by western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR). Colony formation and CCK-8 assays were used to assess cell proliferation, while cell migration and invasion were evaluated by Transwell assay.

Results: FAM172A expression was reduced in breast cancer cells and tissues when compared to controls (p < 0.001). Overexpression of FAM172A repressed proliferation of breast cancer cells (p < 0.001) and suppressed cell migration and invasion (p < 0.001). Overexpression of FAM172A increased Notch 3 expression in breast cancer cells. Knockdown of Notch 3 attenuated the FAM172A overexpression-induced decrease in breast cancer cell proliferation. Silencing of Notch 3 reversed the FAM172A (p < 0.001).

Conclusion: FAM172A represses breast cancer cell growth and metastasis via upregulation of Notch 3; thus, FAM172A and Notch 3 are potential targets for breast cancer therapy.

Keywords: FAM172A, Notch 3, Breast cancer, Cell growth, Metastasis