Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Fatty acid constituents and anticancer activity of Cladophora fracta (OF Müller ex Vahl) Kützing

Tunay Karan¹ , Ramazan Erenler²

¹Department of Molecular Biology and Genetics; ²Department of Chemistry, Faculty of Arts and Sciences, Tokat Gaziosmanpasa University, 60240 Tokat, Turkey.

For correspondence:- Tunay Karan Email: biyo_tunay@hotmail.com

Accepted: 22 September 2018 Published: 31 October 2018

Citation: Karan T, Erenler R. Fatty acid constituents and anticancer activity of Cladophora fracta (OF Müller ex Vahl) Kützing. Trop J Pharm Res 2018; 17(10):1977-1982 doi: 10.4314/tjpr.v17i10.12

© 2018 The authors.
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Abstract

Purpose: To determine the fatty acid constituents and anticancer effect of Cladohora fracta

Methods: Cladophora fracta (O.F. Müller ex Vahl) Kützing was collected from natural ponds in Tokat, Turkey. Antiproliferative and cytotoxic effects of methanol and hexane extracts of C. fracta were investigated on human colon carcinoma (HT29) and non-tumorigenic African green monkey kidney (Vero) cell lines using BrdU cell proliferation enzyme-linked Immunosorbent assay (ELISA) and lactate dehydrogenase (LDH) test, respectively. The fatty acid composition of hexane extract was analyzed by gas chromatography-mass spectrometry (GC-MS).

Results: Oleic acid, palmitic acid, gamma-linoleic acid and linoleic acid were the main constituents of C. fracta. The methanol extract exhibited strong antiproliferative activity on HT29 and Vero cell lines (p < 0.05). The hexane extract revealed its good antiproliferative activity at high concentrations on both cell lines. Cytotoxicity results showed that both methanol and hexane extract had low effect on HT29 cell at low concentrations.

Conclusion: Due to the strong antiproliferative effect of C. fracta methanol extract on HT29 and Vero cell lines, it has potential anticancer properties and recommended for further development as such.

Keywords: Cladophora fracta, Antiproliferative activity, Anti-cancer, Cytotoxic effect, Fatty acid