Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Genotype and haplotype analysis of ABCB1 at 1236, 2677 and 3435 among Jordanian population

Ola Al-Diab¹ , Al-Motassem Yousef¹, Enas Al Manassrah², Ahmad Masadeh³, Muhammed Olemat³, Hisham Qosa⁴, Athar Kherbash¹, Nailya R Bulatova¹

¹Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, The University of Jordan, Amman; ²Clinical Pharmacy Department, Al-Basheer Hospital, Amman; ³Clinical Pharmacy Department, Royal Medical Services, Amman, Jordan; ⁴Department of Basic Pharmaceutical Science, College of Pharmacy, University of Louisiana at Monroe, Monroe, LA, USA.

For correspondence:- Ola Al-Diab Email: o.diab@ju.edu.jo Tel:+962777486930

Received: 15 July 2014 Accepted: 24 April 2015 Published: 29 June 2015

Citation: Al-Diab O, Yousef A, Manassrah EA, Masadeh A, Olemat M, Qosa H, et al. Genotype and haplotype analysis of ABCB1 at 1236, 2677 and 3435 among Jordanian population. Trop J Pharm Res 2015; 14(6):1013-1019 doi: 10.4314/tjpr.v14i6.11

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the frequencies of important allelic variants and their haplotype frequencies of the gene among Jordanian population and to compare findings with those reported for other ethnic groups.

Methods: Genotyping of ABCB1 (C1236T, G2677T/A and C3435T) was carried out on unrelated healthy Jordanian subjects. Different allelic variants were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The haplotype frequencies of these three SNPs were analyzed and compared them with those of other reported populations. Haplotype frequencies were calculated using Golden Helix Tree software and Linkage disequilibrium was represented by D”.

Results: ABCB1 C3435T allele frequencies for C allele and T allele were 0.57 and 0.43, respectively. For ABCB1 G2677T/A the allele frequencies for G allele, T allele, and A allele were 0.65, 0.32 and 0.0, respectively. As for ABCB C1236T, its allele frequencies were 0.65 for C allele and 0.35 for T allele. C1236T, G2677T/A, and C3435T SNPs were expected to be structured in 8 different haplotypes with G-C-C (37.6.0 %), T-T-T (18.6 %), G-C-T (14.3 %) and T-T-C (12 %) that were most prominent. The haplotype frequency distribution of our study group was found to be significantly different from those of Chinese, Indian, Japanese, African and Caucasian (p < 0.0001) and resemble Ashkenazi Jewish and Slovenian populations (p > 0.05).

Conclusion: In addition to earlier studies, the findings of the current study provide evidence that suggest the use of genetic polymorphisms of ABCB1 SNPs as markers for ethnicity and ancestral origin. The analysis of haplotype and genotype can be useful in identifying the relation between ABCB1 polymorphism, disease susceptibility and drug disposition.

Keywords: Genotype, Allele, MDR1, ABCB1, Polymorphism, Haplotype frequencies