Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Geraniin inhibits bladder cancer cell growth via regulation of PI3K/AKT signaling pathways

Junwei Xu¹, Ning Qin², Yebin Yao³, Tao Chen¹, Wenbo Jiang⁴

¹Department of Traditional Chinese Medicine, Affiliated Hospital of Nantong University; ²Department of Medical Nursing Teaching and Research, Nantong Health College of Jiangsu Province; ³Department of Infectious Diseases, Affiliated Hospital of Nantong University, Nantong City, Jiangsu Province 226001; ⁴Department of Cardiovascular Medicine, Suqian Hospital of Traditional Chinese Medicine, Suqian City, Jiangsu Province 223800, China.

For correspondence:- Wenbo Jiang Email: WenboJiangdgh@163.com Tel:+8652782966829

Accepted: 29 January 2020 Published: 29 February 2020

Citation: Xu J, Qin N, Yao Y, Chen T, Jiang W. Geraniin inhibits bladder cancer cell growth via regulation of PI3K/AKT signaling pathways. Trop J Pharm Res 2020; 19(2):253-257 doi: 10.4314/tjpr.v19i2.6

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: The effect of geraniin on human bladder transitional carcinoma was not clear, this study was thus intended to reveal it and reveal the mechanism.

Methods: T24 cells were treated with 5, 10, and 20 μM of geraniin and the viability and apoptosis of T24 cells were determined using thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometry. The protein expression levels of Cyclin D1, p21, BAL-2, BAX, cleaved caspase-3 and PI3K/AKT pathway were evaluated using western blot.

Results: Geraniin decreased T24 cell viability and induced T24 cell cycle arrest. The proportion of T24 cells in S phase was decreased by geraniin. Besides, geraniin promoted T24 cell apoptosis and regulated PI3K/AKT pathway.

Conclusion: Geraniin appears to regulate bladder cancer cell growth by decreasing the levels of PI3K and AKT phosphorylation. Thus, this agent may be useful in the management of bladder cancer.

Keywords: Geraniin, T24 cells, Apoptosis, PI3K/AKT signaling