Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Green tea polyphenol induces significant cell death in human lung cancer cells

Jie Huang, Fa-jiu Li, Shi Chen, Yi Shi, Xiao-jiang Wang, Chuan-hai Wang, Qing-hua Meng, Cheng Jiang, Zhi-yang Zhu, Cheng-Hong Li

Department of Respiratory Medicine, Wuhan No.6 Hospital, Affiliated Hospital of Jianghan University, Wuhan, Peopleâ€™s Republic of China;

For correspondence:- Cheng-Hong Li Email: lichenghong323@hotmail.com Tel:+862782426502

Received: 26 June 2016 Accepted: 14 April 2017 Published: 30 May 2017

Citation: Huang J, Li F, Chen S, Shi Y, Wang X, Wang C, et al. Green tea polyphenol induces significant cell death in human lung cancer cells. Trop J Pharm Res 2017; 16(5):1021-1028 doi: 10.4314/tjpr.v16i5.8

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the dose–response relationship of green tea polyphenol in an animal model of lung cancer.

Methods: The effects of epigallocatechin-3-gallate (EGCG) on the inhibition of xenograft tumor growth, the accumulation of 8-hydroxy-2'-deoxyguanosine (8-OHdG), and apoptosis based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were evaluated in non-small cell lung cancer (NSCLC) cell lines, namely, H1155, H661, and A427 (a human lung carcinoma-derived cell line). The dose-dependent effects of EGCG on H1155 xenograft tumor growth, as well as the levels of EGCG in plasma and tissue, were also determined in male nude mice.

Results: EGCG inhibited the growth of NSCLC-derived cell lines (H1155) over a 45-day period. There was a significant reduction (57 %) in tumor weight in EGCG-fed (0.5 %) animals compared with the control group (p < 0.05). Linear regression analysis revealed a dose-dependent reduction in tumor size. MTT assay results revealed inhibition of H1155 cell growth (25 %, p < 0.05) after 24 h treatment with EGCG. The addition of superoxide dismutase (5 U/mL) and catalase (30 U/mL) reduced the inhibitory effect of EGCG. Mice administered 30 mg/kg EGCG via intraperitoneal injection exhibited the least amount of oxidative stress.

Conclusion: The results demonstrate the concentration-dependent inhibitory effects of EGCG on lung cancer cells, including H1155 cells, both in vitro and in vivo. The induction of reactive oxygen species, oxidative DNA damage, and apoptosis were evident following EGCG treatment

Keywords: Green tea, Lung cancer, Catechins, Epigallocatechin-3-gallate, Oxidative stress, Oxidative DNA damage