Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

High Performance Liquid Chromatographic Determination of Phenolic Compounds in Propolis

Li Yang^1,3, Qing-Hua Yan², Jin-You Ma¹, Qing Wang¹, Jian-Wei Zhang¹, Guo-Xi Xi³

¹Department of Experimental Center, Henan Institute of Science and Technology; ²Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003; ³School of Environment, Henan Normal University. Key Laboratory for Yellow River and Huai River Water Environment and Pollution ControlA292;Ministry of Education. Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan 453007, PR China.

For correspondence:- Guo-Xi Xi Email: syzxhome@163.com Tel:+863733040746

Received: 6 November 2012 Accepted: 10 June 2013 Published: 18 October 2013

Citation: Yang L, Yan Q, Ma J, Wang Q, Zhang J, Xi G. High Performance Liquid Chromatographic Determination of Phenolic Compounds in Propolis. Trop J Pharm Res 2013; 12(5):771-776 doi: 10.4314/tjpr.v12i5.17

© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To develop a simple, precise and rapid high-performance liquid chromatographic technique coupled with photodiode array detection (DAD) method for the simultaneous determination of rutin, quercetin, luteolin, genistein, galangin and curcumin in propolis.

Methods: Ultrasound-assisted extraction was applied to isolate six antioxidant phenolic compounds from propolis samples. Chromatographic separation was performed using an Eclipse XDB-C18 column (150 mm×4.6 mm, 5 μm) by isocratic elution at 25 °C. The mobile phase consisted of phosphate buffered saline (pH = 4.5) and methanol (40/60, v/v). The flow rate was set at 0.8 ml/min and the effluent was monitored using a DAD set at 260 nm.

Results: Excellent linear correlation coefficient was observed in the range of 0.9977 to 0.9997. The reproducibility of the method was 1.58 % (RSD) and accuracy was confirmed with average recovery ranging from 95.71 to 104.26 %. Six antioxidant phenolic compounds in propolis samples were successfully determined within 50 min.

Conclusion: The proposed isocratic LC method gave good separation and accurate determination of rutin, quercetin, luteolin, genistein, galangin and curcumin in propolis. The proposed method showed good sensitivity, linearity, repeatability and accuracy.

Keywords: Propolis, Rutin, Quercetin, Luteolin, Genistein, Galangin, Curcumin, HPLC