Li Yang1,3,
Qing-Hua Yan2,
Jin-You Ma1,
Qing Wang1,
Jian-Wei Zhang1,
Guo-Xi Xi3
1Department of Experimental Center, Henan Institute of Science and Technology;
2Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003;
3School of Environment, Henan Normal University. Key Laboratory for Yellow River and Huai River Water Environment and Pollution ControlA292;Ministry of Education. Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan 453007, PR China.
For correspondence:- Guo-Xi Xi
Email: syzxhome@163.com Tel:+863733040746
Received: 6 November 2012
Accepted: 10 June 2013
Published: 18 October 2013
Citation:
Yang L, Yan Q, Ma J, Wang Q, Zhang J, Xi G.
High Performance Liquid Chromatographic Determination of Phenolic Compounds in Propolis. Trop J Pharm Res 2013; 12(5):771-776
doi:
10.4314/tjpr.v12i5.17
© 2013 The authors.
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Abstract
Purpose: To develop a simple, precise and rapid high-performance liquid chromatographic technique coupled with photodiode array detection (DAD) method for the simultaneous determination of rutin, quercetin, luteolin, genistein, galangin and curcumin in propolis.
Methods: Ultrasound-assisted extraction was applied to isolate six antioxidant phenolic compounds from propolis samples. Chromatographic separation was performed using an Eclipse XDB-C18 column (150 mm×4.6 mm, 5 μm) by isocratic elution at 25 °C. The mobile phase consisted of phosphate buffered saline (pH = 4.5) and methanol (40/60, v/v). The flow rate was set at 0.8 ml/min and the effluent was monitored using a DAD set at 260 nm.
Results: Excellent linear correlation coefficient was observed in the range of 0.9977 to 0.9997. The reproducibility of the method was 1.58 % (RSD) and accuracy was confirmed with average recovery ranging from 95.71 to 104.26 %. Six antioxidant phenolic compounds in propolis samples were successfully determined within 50 min.
Conclusion: The proposed isocratic LC method gave good separation and accurate determination of rutin, quercetin, luteolin, genistein, galangin and curcumin in propolis. The proposed method showed good sensitivity, linearity, repeatability and accuracy.
Keywords: Propolis, Rutin, Quercetin, Luteolin, Genistein, Galangin, Curcumin, HPLC