Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

IFI6 predicts prognosis and promotes cell growth of human colorectal cancer

Chu Lv¹, Meng Li²

¹Department of Oncology, Traditional Chinese Medical Hospital of Zhuji, Shaoxing, Zhejiang Province 311800, China; ²Department of General Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050000, China.

For correspondence:- Meng Li Email: limeng861126@163.com Tel:+8631166002904

Accepted: 27 January 2023 Published: 27 February 2023

Citation: Lv C, Li M. IFI6 predicts prognosis and promotes cell growth of human colorectal cancer. Trop J Pharm Res 2023; 22(2):245-251 doi: 10.4314/tjpr.v22i2.4

© 2023 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To analyze the effect of IFI6 in colorectal cancer (CRC).

Methods: Differentially-expressed genes were analyzed using a volcano plot. Metascape and STRING were used for functional enrichment and protein-protein interaction network analyses, respectively. expression of IFI6 was analyzed using the online platforms TIMER, UALCAN, and GEPIA. The correlation between IFI6 and poor prognosis was analyzed using prognoscan, while Western blotting was used to determine the expressions of related proteins. Cell proliferation was performed by CCK8 and foci formation assays. Cell apoptosis was assessed using flow cytometry.

Results: Differentially-expressed genes between CRC cells and alisertib-resistant CRC cells comprised 84 upregulated genes and 358 downregulated genes (p < 0.05). Enrichment analysis showed that differential genes were mainly involved in interferon α/β signaling, antiviral mechanism of interferon-stimulated genes, and positive regulation of the immune response. IFI6, a hubgene in the protein-protein interaction network, was highly expressed in CRC (p < 0.001) and correlated with poor prognoses (p = 0.028). IFI6 knockdown inhibited cell viability and colony formation and induced cell apoptosis by upregulation of p53, p21, and Bax.

Conclusion: These results suggest that IFI6 may be a biomarker for the diagnosis of human CRC.

Keywords: IFI6, Colorectal cancer, Cell viability, Immune infiltration, Poor prognosis