Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Idala: An unnamed Function Peptide Vaccine for Tuberculosis

Salmah Ismail¹, Nadiya Taha Darwish¹, Teow Chong Teoh¹ , Mahmood Ameen Abdulla², Rahmat Omar³

¹Institute of Biological Sciences, Science Faculty; ²Department of Molecular Medicine; ³Department of Otorhinolaryngology, Medical Faculty, University of Malaya, 50603 Kuala Lumpur, Malaysia.

For correspondence:- Teow Teoh Email: ttchong@um.edu.my Tel:+60379677189

Received: 10 May 2012 Accepted: 24 October 2012 Published: 13 December 2012

Citation: Ismail S, Darwish NT, Teoh TC, Abdulla MA, Omar R. Idala: An unnamed Function Peptide Vaccine for Tuberculosis. Trop J Pharm Res 2012; 11(6):879-886 doi: 10.4314/tjpr.v11i6.2

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate Myt272 protein antigenicity and immunogenicity by trial vaccination in mice and its in silico analysis as a potential peptide vaccine for tuberculosis.

Methods: Myt272 gene, which has 100 % identity with Mycobacterium tuberculosis H37Rv unknown function gene Rv3424c, was ligated by genomic shotgun approach into the expression vector pQE32, and transformed into Escherichia coli SG13009. expression during cell growth was induced by isopropyl-β-D-thiogalactopyranoside. The recombinant protein was isolated from the harvested cell lysate and injected in mice for immunogenicity experiment up to 42 days. ELISA tests with anti-His antibodies were performed on the collected individual blood samples’ sera. Color development in a microplate reader was measured at 450 nm.

Results: The protein was predicted to have a mass of approximately 13 kDa and was present in the soluble fraction of the cell lysate. The immunogenicity test on Myt272 protein revealed very statistically significant high levels of antibodies detected by ELISA in the sera of immunized group of mice compared to negative controls.

Conclusion: A 10.1 kD unnamed function (IDALA) protein from Rv3424 gene could be the potential peptide vaccine for tuberculosis tested by mice immunogenicity experiment.

Keywords: Tuberculosis H37Rv, Myt272 clone, IDALA, Immunogenicity tests, In silico study