Hong Xiang Li1,2,
Yan Qiu Wang3,
Jin Shuang Zhao3,
Li Xia Zhu2,
Wen Ying Huai3,
Rong Qiang Liu3,
Tian E Zhang3 
,
Yun Deng2
For correspondence:- Tian Zhang Email: zhte2003@aliyun.com
Accepted: 29 August 2019 Published: 01 October 2019
Citation: Li HX, Wang YQ, Zhao JS, Zhu LX, Huai WY, Liu RQ, et al. Immunosuppressive effect of voacamine from Voacanga africana Stapf based on SPRi experiment. Trop J Pharm Res 2019; 18(9):1889-1893 doi: 10.4314/tjpr.v18i9.15
© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the affinity of a bis-indole alkaloid - voacamine from Voacanga Africana Stapf for IL-2Rα - and its immunosuppressive effect on concanavalin A-induced T cell proliferation and lipopolysaccharide -induced B cell proliferation in vitro.
Methods: Surface plasmon resonance imaging (SPRi) was used to screen the target protein of voacamine, while CCK-8 kit was used to evaluate cytotoxicity. Mitogen-induced proliferation assay was carried out to assess the inhibitory effect of voacamine on Con A-induced T cell proliferation and LPS-induced B cell proliferation. The binding characteristics of voacamine were investigated using a binding model with IL-2Rα constructed based on molecular docking simulation.
Results: Voacamine had a high-affinity for IL-2Rα with an equilibrium dissociation constant (KD) of 1.85×10-8 M. Cytotoxicity data showed that voacamine did not exhibit cytotoxicity at concentrations lower than 0.32 µM. However, it exerted significant immunosuppressive effect on B cells at a lower concentration, but had no influence on proliferation of T cells. Autodock results indicate that voacamine has a good interaction with the enzyme active site.
Conclusion: Voacamine and its analogues exert influence on the immune system.
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