Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In-vitro cytotoxicity of biosynthesized gold nanoparticles against thyroid cancer cell lines

Yuan-Mei Li , Yuan-Yuan Wang, Bo-Ning Cheng

Department of Endocrinology, Xixi Hospital of Hangzhou CITY, Hangzhou 310023, Zhejiang, China;

For correspondence:- Yuan-Mei Li Email: liyuanmeiym@hotmail.com

Received: 13 July 2016 Accepted: 7 June 2017 Published: 31 July 2017

Citation: Li Y, Wang Y, Cheng B. In-vitro cytotoxicity of biosynthesized gold nanoparticles against thyroid cancer cell lines. Trop J Pharm Res 2017; 16(7):1523-1528 doi: 10.4314/tjpr.v16i7.9

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To undertake the biosynthesis of gold nanoparticles (AuNPs) using Shorea tumbuggaia bark extract and to study their in-vitro cytotoxicity in thyroid cancer (SW579) cell lines.

Methods: AuNPs were prepared by adding 10 mL of Shorea tumbuggaia extract to 5 mL of 2 × 10^-3 M of chloroauric acid (HAuCl₄) and stirred at room temperature for about 20 min. The AuNPs were evaluated by x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible (UV–Vis) spectroscopy and transmission electron microscopy (TEM). They were also assessed for cytotoxicity against SW579 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

Results: The reaction of Shorea tumbuggaia extract with HAuCl₄ led to a change in the color of the reaction solution to ruby red after 20 min, indicating the formation of AuNPs. The results of various instrumental tests, including XRD, TEM, UV–vis spectroscopy and FT-IR spectroscopy confirmed the formation of AuNPs. TEM images showed spherical NPs with a mean particle size of 20 nM. Further, in-vitro cytotoxicity results indicated concentration-dependent cytotoxicity against SW579 cell lines.

Conclusions: A simple, green and low-cost method for the preparation of AuNPs using Shorea tumbuggaia extract has been achieved. The AuNPs exert cytotoxic activity against SW579 cell lines.

Keywords: Shorea tumbuggaia, Polyphenols, Gold nanoparticles, Cytotoxicity, Thyroid cancer cell