Ihda Dian Kusuma1,2
,
Aris Rosidah1,3,
Eviana Norahmawati2,
Nurdiana 3,
Agustina Tri Endharti5
1Biomedical Magister Program, Faculty of Medicine, Universitas Brawijaya;
2Department of Pathology Anatomy, Faculty of Medicine, Universitas Brawijaya;
3Department of Pharmacology, Faculty of Medicine, Islamic University of Malang;
4Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya;
5Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Indonesia.
For correspondence:- Ihda Kusuma
Email: ihdadiankusuma@ymail.com
Accepted: 18 June 2018
Published: 28 July 2018
Citation:
Kusuma ID, Rosidah A, Norahmawati E, N, Endharti AT.
Indonesian Pasuruan propolis extract does not exert anti-proliferation and pro-apoptotic effect on human colon carcinoma cell line HT-29. Trop J Pharm Res 2018; 17(7):1345-1351
doi:
10.4314/tjpr.v17i7.17
© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Abstract
Purpose: To evaluate the anti-cancer activity of Indonesian Pasuruan propolis extract (PPE) against HT-29 human colon carcinoma cell line.
Methods: HT-29 cells were cultured and treated in different concentrations of PPE (50, 100, 200, 400 µg/mL) for 24 h. The cells were evaluated by several indicators such as cell proliferation and apoptosis as well as the expression of protein Ki67, p53, cyclin D1, and Bcl-xL.
Results: Administration of PPE inhibited cell proliferation of HT-29 in concentration-dependent manner but the effect was not significant (p = 0.842); the results were similar with regard to the expression of Ki67 in HT-29 cells (p = 0.953). Administration of 400 µg/mL PPE insignificantly decreased cyclin D1 expression (p = 0.149). The concentration of PPE at 50, 100, and 200 µg/mL induced cell apoptosis of HT-29 cells initially, but the level of apoptosis subsequently decreased (p = 0.416). Furthermore, the expressions of p53 and Bcl-xL decreased following treatment with PPE at 50, 100 and 200 µg/mL but increased for the PPE 400 µg/mL group (p = 0.000).
Conclusion: PPE reduced the expressions of p53, Ki67, cyclin D1, and Bcl-xL insignificantly as it it generally failed inhibit cell proliferation and promote cell apoptosis of HT-29 cells.
Keywords: Bcl-xL, Colon cancer, Cyclin D1, Human colon adenocarcinoma cell line HT-29, Proliferation marker Ki67, Tumor protein p53