Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Influence of miR155 on allergic conjunctivitis in mice via regulation of NF-κB signal pathway

Mengtian Bai^1-5, Yan Li^1-5, Yun Li^1-6, Zhulin Hu^1-5

¹Department of Ophthalmology, Fourth Affiliated Hospital of Kunming Medical University; ²Yunnan Eye Institute; ³Key Laboratory of Yunnan Province for the Prevention and Treatment of Ophthalmology; ⁴Provincial Innovation Team for Cataract and Ocular Fundus Disease, The Second People's Hospital of Yunnan Province; ⁵Expert Workstation of Yao Ke; ⁶Department of Oncology, First Affiliated Hospital of Kunming Medical University, Kunming, China.

For correspondence:- Zhulin Hu Email: ceby54@163.com

Accepted: 28 July 2019 Published: 27 August 2019

Citation: Bai M, Li Y, Li Y, Hu Z. Influence of miR155 on allergic conjunctivitis in mice via regulation of NF-κB signal pathway. Trop J Pharm Res 2019; 18(8):1603-1608 doi: 10.4314/tjpr.v18i8.5

© 2019 The authors.
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Abstract

Purpose: To investigate the effect of miR-155 on allergic conjunctivitis (AC) in mice, and to elucidate the mechanism of action.

Methods: Sixty (60) Balb/c mice were randomly divided into three groups with 20 mice per group. Ovalbumin (OVA) was used to induce experimental model of AC in mice. Mice in the AC+miR-155 siRNA group were given miR-SiRNA once daily for 2 weeks before inducing AC. The expressions of miR-155 in conjunctival tissue of the control and AC groups were assayed with reverse transcription-polymerase chain reaction (RT-PCR). In addition, anti-OVA IgE antibody, eotaxin, IL-13 and IFN-γ levels were determined using ELISA (enzyme-linked immunosorbent assay). The regulatory effect of miR-155 on the NF-κB signal pathway in mice conjunctiva tissue with AC was determined using immunoblotting.

Results: Higher miR-155 expression was seen in serum of AC group than in that of control group (p < 0.05). Inhibition of miR-155 mitigated AC-induced pathological injury, reduced infiltration of eosinophils, lowered serum levels of anti-AVO IgE antibody eotaxin and Il-13, and increased IFN-γ level (p < 0.05). Phosphorylation of P65 of conjunctiva tissue of AC mice was blocked after inhibition of miR-155.

Conclusion: The inhibition of miR-155 ameliorates AC in mice most likely via a mechanism related to the inhibition of phosphorylation of P65. This provides a theoretical basis for new drug research and development.

Keywords: Allergic conjunctivitis, Mice, miR-155, Phosphorylation of P65, Ovalbumin