Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Inhibition of Pro-inflammatory Cytokines by Ethyl Acetate Extract of Scrophularia striata

Abbas Azadmehr¹ , Ghorban Maliji² , Reza Hajiaghaee³, Mojtaba Shahnazi⁴, Ahmad Afaghi⁵

¹Immunology Department, Qazvin University of Medical Sciences, Qazvin; ²Immunology Department, Babol University of Medical Sciences, Babol; ³Department of Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran; ⁴Parasitology Department; ⁵Social Medicine Department, Qazvin University of Medical Sciences, Qazvin, Iran.

For correspondence:- Ghorban Maliji Email: ghmaliji@yahoo.com Tel:+9811122295915

Received: 7 November 2011 Accepted: 15 October 2012 Published: 13 December 2012

Citation: Azadmehr A, Maliji G, Hajiaghaee R, Shahnazi M, Afaghi A. Inhibition of Pro-inflammatory Cytokines by Ethyl Acetate Extract of Scrophularia striata. Trop J Pharm Res 2012; 11(6):893-897 doi: 10.4314/tjpr.v11i6.4

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of ethyl acetate extract of S. striata on pro-inflammatory production by macrophages.

Methods: Mouse peritoneal macrophages were cultured in solvent either alone or with 2 µg/ml lipopolysaccaride (LPS) with/without different doses of ethyl acetate extract of S. striata. Production of pro-inflammatory cytokines including interleukin 1β (IL-1β), tumor necrosis factor - α (TNF-α) and prostaglandin E2 (PGE2) were examined using ELISA.

Results: Ethyl acetate fraction of S. striata in doses of 10, 50, 100 and 200 µg/ml significantly (p < 0.05) inhibited pro-inflammatory mediators (IL-1β, TNF-α, and PGE2) production by LPS stimulated peritoneal macrophages.

Conclusion: The anti-inflammatory effect of the extract on pro-inflammatory cytokines may ameliorate inflammatory diseases, possibly via an immunomodulatory mechanism.

Keywords: Pro-inflammatory, Cytokines, Ethyl acetate extract, Scrophularia striata