Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Inhibitory effect and mechanism of action of tanshinone IIA on human bladder cancer cell J82

Xianjun Zhang, Ziwen Lu, Songzhe Piao, Tao Hong

Department of Urology, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, Zhejiang Province, PR China;

For correspondence:- Tao Hong Email: taohongtz@aliyun.com

Accepted: 19 March 2021 Published: 30 April 2021

Citation: Zhang X, Lu Z, Piao S, Hong T. Inhibitory effect and mechanism of action of tanshinone IIA on human bladder cancer cell J82. Trop J Pharm Res 2021; 20(4):771-774 doi: 10.4314/tjpr.v20i4.16

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the therapeutic influence of tanshinone IIA on human bladder cancer cell J82, and the possible signal route involved.

Methods: Cell proliferative potential was measured using MTT assay, while the expressions of associated genes were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunoblot assays.

Results: Tanshinone IIA decreased J82 cell survival rate by > 42 % and inactivated apoptosis by suppressing PI3K/AKT/mTOR signal route. Moreover, it decreased Bcl-2, but upregulated caspase and Bax (p < 0.05).

Conclusion: The inhibitory effect of TIIA on human bladder cancer suggests that TIIA can be developed into an anti-tumor agent.

Keywords: Tanshinone IIA, Human bladder cancer cell J82, Caspase-3