Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Interleukin-34 protects against sepsis in mice by regulating CXCL1/CCL2 immune response

Jianjun Wang¹, Feng Yang², Yuanyuan Bu¹, Jinghong Jia¹, Aibin Cheng¹, Jihua Zhao⁴

¹Department of Intensive Medicine; ²Department of Neurosurgery; ³Department of Anesthesiology, North China University of Science and Technology Affiliated Hospital; ⁴North China University of Science and Technology School of Clinical Medicine, Tangshan City, China.

For correspondence:- Jihua Zhao Email: bgfr37@163.com

Accepted: 6 March 2021 Published: 31 March 2021

Citation: Wang J, Yang F, Bu Y, Jia J, Cheng A, Zhao J. Interleukin-34 protects against sepsis in mice by regulating CXCL1/CCL2 immune response. Trop J Pharm Res 2021; 20(3):525-530 doi: 10.4314/tjpr.v20i3.12

© 2021 The authors.
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Abstract

Purpose: To study the protective effect of interleukin-34 (IL-34) against sepsis in mice, and the mechanism involved.

Methods: Ninety healthy male mice were selected and assigned to sham, model and recombinant IL-34 protein groups. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were assayed. Moreover, histopathological changes in lung, liver and kidney were recorded, and levels of C-X-C Motif Chemokine Ligand 1 (CXCL1) and C-C Motif Chemokine Ligand 2 (CCL2) in each group of mice were measured.

Results: Peritoneal lavage fluid and serum concentrations of AST, ALT, LDH, CXCL1 and CCL2 were significantly elevated, relative to sham mice (p < 0.05). Mice survival in the drug group was markedly increased from day 1 to day 5; also, serum ALT, LDH and AST were significantly reduced, while CXCL1 and CCL2 concentrations in serum and peritoneal lavage fluid were increased, relative to model mice (p < 0.05).

Conclusion: IL-34 improves survival of septic mice by inducing CXCL1/CCL2 immune response, resulting in a protective effect on the airway. Thus, the CXCL1/CCL2 pathway mediated by IL-34 may be useful in the development of drugs for the treatment of sepsis.

Keywords: Interleukin-34, Sepsis, CXCL1/CCL2, Protective effect