Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Investigation of antibacterial and anti-cancer activities of Streptomyces sp SRF1 culture filtrate

Kusavadee Sangdee¹, Benjaporn Buranrat¹, Prapairat Seephonkai^2,3, Nilawan Surapong², Aphidech Sangdee^4,5

¹Biomedical Sciences Research Unit, Faculty of Medicine, Mahasarakham University, Muang District, Maha Sarakham 44000; ²Nano Technology Research Unit; ³Center of Excellence for Innovation in Chemistry (PERCH-CIC), Department of Chemistry, Faculty of Science, Mahasarakham University, Khamriang Sub-District, Kantarawichai District, Maha Sarakham 44150; ⁴Department of Biology; ⁵Microbiology and Applied Microbiology Research Unit, Faculty of Science, Mahasarakham University, Kantarawichai District, Maha Sarakham 44150, Thailand.

For correspondence:- Aphidech Sangdee Email: aphidech.s@msu.ac.th

Accepted: 5 October 2017 Published: 30 November 2017

Citation: Sangdee K, Buranrat B, Seephonkai P, Surapong N, Sangdee A. Investigation of antibacterial and anti-cancer activities of Streptomyces sp SRF1 culture filtrate. Trop J Pharm Res 2017; 16(11):2727-2734 doi: 10.4314/tjpr.v16i11.21

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the antibacterial activity and cytotoxic effects of Streptomyces sp. SRF1 culture filtrate extract against breast cancer cell line.

Methods: The activity of the extract against Gram-positive and Gram-negative bacteria was initially screened by an agar-well diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were measured by broth microdilution method. Time-kill assays were also performed, and extract-induced morphological and ultrastructural changes to bacterial cells were investigated. Sulforhodamine B (SRB) assay was performed to determine the cytotoxicity of the extract against the human breast cancer cell line, MCF-7.

Results: Antibacterial activity by the extract was detected against four strains of Gram-positive pathogens including one strain of methicillin-susceptible Staphylococcus aureus (MSSA) and 3 strains of methicillin-resistant Staphylococcus aureus (MRSA) - with low MIC and MBC values. This activity was bactericidal after 6 h exposure. Morphological alterations were detected on the cell surface of both MSSA and MRSA. The extract also inhibited MCF-7 cell growth with half-maximal concentration (IC₅₀) of 211.67 ± 33.95 µg/mL in 72 h.

Conclusions: Streptomyces sp. SRF1 culture filtrate extract exhibits potent antibacterial and anticancer activities and thus, represents a potential source of antibacterial and anticancer drugs

Keywords: Antibacterial activity, Anti-breast cancer, Staphylococcus aureus, Streptomyces sp. SRF1