Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Investigation of the Antiasthmatic Properties of Ethanol Extract of Callophyllis japonica in Mice

Won Sun Park¹, Kyung Soo Lee², Jin Ho Chun², Sang Hwa Urm², Dae-Sung Lee³, Da-Young Lee⁴, Sae-Gwang Park⁴, Su-Kil Seo⁴, Soo-Jin Heo⁵, Zhong-ji Qian⁶, Won-Kyo Jung⁶, Il-Whan Choi⁴

¹Department of Physiology, Kangwon National University School of Medicine, Chuncheon; ²Department of Preventive Medicine, College of Medicine, Inje University, Busan; ³POSTECH Ocean Science and Technology Institute, Pohang University of Science and Technology, Pohang; ⁴Department of Microbiology, College of Medicine, Inje University, Busan; ⁵Marine Living Resources Research Department, Korea Ocean Research and Development Institute, Ansan; ⁶Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University, Republic of Korea.

For correspondence:- Il-Whan Choi Email: cihima@inje.ac.kr Tel:+82518906461

Received: 2 February 2013 Accepted: 10 October 2013 Published: 24 December 2013

Citation: Park WS, Lee KS, Chun JH, Urm SH, Lee D, Lee D, et al. Investigation of the Antiasthmatic Properties of Ethanol Extract of Callophyllis japonica in Mice. Trop J Pharm Res 2013; 12(6):981-987 doi: 10.4314/tjpr.v12i6.17

© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine whether an ethanol extract from Callophyllis japonica (C. japonica) could attenuate indices of airway inflammation in a murine model of ovalbumin (OVA)-induced asthma.

Methods: The free radical scavenging activity of the C. japonica ethanol extracts (CJE) were investigated using an electron spin resonance (ESR) system. To make develop animal model of asthma, mice were sensitized and challenged with OVA.

Results: CJE exhibited considerable scavenging activity of 71.08 ± 0.73, 79.11 ± 6.04%, 75.95 ± 7.01%, and 48.56 ± 5.96% of DPPH, alkyl, superoxide, and hydroxyl radicals, respectively. The successive intraperitoneal administration of CJE reduced the number of eosinophils in bronchoalveolar lavage (BAL) fluid, development of airway hyperresponsiveness (AHR), an increase in pulmonary Th2 cytokines, and allergen-specific immunoglobulin E (IgE).

Conclusion: Administration of CJE markedly alleviates all indices of airway inflammation. This study provides evidence that CJE plays a critical role in the amelioration of the pathogenetic process of allergic asthma in mice.

Keywords: Asthma, Phenolic contents, Free radical scavenging, Airway hyper-responsiveness, Cytokines, Immunoglobulin E