Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Isolation and Characterization of Antithrombin Peptides from the Saliva of Malaysian Leeches

Mohamed Alaama¹, Helal ABM Uddin¹ , Abbas Mohammad Ghawi², Ahmed Merzouk³, Abdualrahman Mohammed Abdualkader¹, Mohamed bin Awang⁴

¹Department of Pharmaceutical Chemistry; ²Department of Basic Medical Science, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Malaysia; ³Biopep Solutions Inc, Vancouver, BC, Canada; ⁴Faculty of Pharmacy, CUCMS, Cyberjaya, Selangor.

For correspondence:- Helal Uddin Email: abmhelal@iium.edu.my

Received: 11 May 2013 Accepted: 23 February 2014 Published: 23 April 2014

Citation: Alaama M, Uddin HA, Ghawi AM, Merzouk A, Abdualkader AM, Awang Mb. Isolation and Characterization of Antithrombin Peptides from the Saliva of Malaysian Leeches. Trop J Pharm Res 2014; 13(4):553-557 doi: 10.4314/tjpr.v13i4.10

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To isolate and characterize the antithrombin compounds of Malaysian leeches’ saliva collection (LSC) for use as anticoagulant proteins and peptides.

Method: Reversed phase - high performance liquid chromatography (RP-HPLC) was used to isolate all proteins from LSC. All isolated proteins were tested for anticoagulant activity by applying two tests, thrombin time test and the inhibition of the amidolytic activity of thrombin on the chromogenic substrate (S-2238). The molecular weights of isolated active compounds were identified using tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE).

Results: More than 40 proteins were isolated from LSC using RP-HPLC. Two compounds (protein 1 and protein 2) were found to be active as they increased thrombin time by 30.26 and 36.75%, respectively and inhibited the amidolytic activity of thrombin. as evaluated by measuring the conversion of the chromogenic substrate (S-2238). Decrease in the conversion of the substrate (S-2238) by 31.10 and 41.61%, respectively was observed. The molecular weights of the proteins (6.289 and 14.255 kDa, respectively) had no similarity with other leech species.

Conclusion: Two antithrombin proteins with anticoagulant properties were isolated from the saliva of Malaysian leeches using one-step isolation procedure. The proteins did not show any similarity to previous isolated proteins.from leech.

Keywords: Leech, Saliva, Antithrombin, Anticoagulant, Peptides, Molecular weight