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Original Research Article | OPEN ACCESS

Isolation and Morphological Characterization of Antibiotic Producing Actinomycetes

Nanjwade K Basavaraj1 , S Chandrashekhara2, Ali M Shamarez1, Prakash S Goudanavar2, Fakirappa V Manvi1

1KLES's College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka; 2KLES's College of Pharmaceutical Sciences, Nipani, Karnataka, India.

For correspondence:-  Nanjwade Basavaraj   Email: bknanjwade@yahoo.co.in   Tel:+918312471399

Received: 12 March 2009        Accepted: 21 April 2010        Published: 24 June 2010

Citation: Basavaraj NK, Chandrashekhara S, Shamarez AM, Goudanavar PS, Manvi FV. Isolation and Morphological Characterization of Antibiotic Producing Actinomycetes. Trop J Pharm Res 2010; 9(3):231-236 doi: 10.4314/tjpr.v9i3.3

© 2010 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To isolate and characterize antibiotic producing actinomycetes from soil samples in Belgaum, Karnataka, India.
Methods: Crowded plate technique was used for the isolation of actinomycetes in media such as soybean – casein digest medium and actinomycetes isolation agar. The morphological and cultural characterization of one of the selected strains, designated A-4, was performed as per International Streptomycete Project (ISP).
Results: Morphological and cultural studies showed that A-4 belonged to the Actinomycete genus. The morphological and cultural characteristics of the A-4 mutant showed cellular and aerial growth as well as soluble pigment formation in various ISP media.
Conclusion: Findings from this investigation revealed that the selected strain, A-4, is an actinomycete.

Keywords: Actinomycete, Antibiotic, MIC, Crowded plate technique, Morphology

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