Ying Liu1,2,3,
Xin Chao Liu1,
Qi Yong Liu2,
Chang Niu3,
Zhi Long Liu1
1Department of Entomology, China Agricultural University;
2Yuanmingyuan West Road, Haidian District, Beijing 100193, 2State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206;
3College of Life Sciences, Capital Normal University, Haidian District, Beijing 100048, China.
For correspondence:- Zhi Liu
Email: zhilongliu@cau.edu.cn Tel:+86-10-62732800
Received: 2 August 2014
Revised: 15 December 2014
Published: 30 January 2015
Citation:
Liu Y, Liu XC, Liu QY, Niu C, Liu ZL.
Larvicidal activity of Illicium difengpi BN Chang (Schisandraceae) stem bark and its constituent compounds against Aedes aegypti L. Trop J Pharm Res 2015; 14(1):103-109
doi:
10.4314/tjpr.v14i1.15
© 2015 The authors.
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Abstract
Purpose:To determine the larvicidal activity of the essential oil derived from Illicium difengpi B.N. Chang stem bark (Schisandraceae) and its major constituents against the larvae of Aedes aegypti L.
Methods:Essential oil of I. difengpi stem bark was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mas spectrometry (GC-MS). The activity of the essential oil and its major constituents was evaluated, using World Health Organization (WHO) procedures, against the fourth instar larvae of A. aegypti for 24 h, and larval mortalities recorded at essential oil/compound concentrations ranging from 6.0 - 200 μg/mL.
Results:A total of 36 components of the essential oil of I. difengpi were identified. The principal compounds are safrole (18.21 %), linalool (13.47 %), 1,8-cineole (12.84 %), and myristicin (8.06 %) followed by α-terpineol (4.77 %), β-pinene (4.45 %) and 4-terpineol (4.38 %). The essential oil exhibited larvicidal activity against A. aegypti with LC50 (median lethal concentration) of 31.68 μg/mL. The major constituents, myristicin, safrole, and 1, 8-cineole, exhibited LC50 of 15.26, 39.45, and 72.18 μg/mL, respectively.
Conclusion:The findings obtained indicate that the essential oil of I. difengpi and its major constituents have potentials for use in the control of A. aegypti larvae and may therefore be useful in the search for newer, safer and more effective natural compounds as larvicides.
Keywords: Illicium difengpi, Aedes aegypti, Larvicidal activity, Myristicin, Safrole, 1,8-Cineole, Linalool