Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Lidocaine, an anesthetic drug, protects Neuro2A cells against cadmium toxicity

Peng Chen, Wenyu Zhang, Xuefeng Li, Longyun Li

Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, China;

For correspondence:- Longyun Li Email: VMaciveragook@yahoo.com Tel:+8643184997222

Accepted: 18 April 2019 Published: 31 May 2019

Citation: Chen P, Zhang W, Li X, Li L. Lidocaine, an anesthetic drug, protects Neuro2A cells against cadmium toxicity. Trop J Pharm Res 2019; 18(5):1033-1039 doi: 10.4314/tjpr.v18i5.17

© 2019 The authors.
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Abstract

Purpose: To investigate the neuroprotective effect of lidocaine in Neuro2A cells

Methods: Differentiated N2a cells were used in this study. Cell viability and neuroprotection were assessed using dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypan blue assays, while Bax/Bcl-2 expression was assayed by western blotting. Mitochondrial membrane potential, reactive oxygen species and calcium levels were measured using flow cytometry.

Results: Lidocaine protected differentiated N2a cells against cadmium-induced toxicity, and also attenuated cadmium toxicity-induced changes in mitochondrial membrane potential (MMP), reactive oxygen species (ROS) and calcium (Ca²⁺) levels. Furthermore, Bax/Bcl-2 ratio, which was disrupted by cadmium, and cadmium-induced apoptosis, were reversed by lidocaine.

Conclusion: Lidocaine protects differentiated N2a cells against cadmium-induced toxicity by reversing apoptosis. Thus, lidocaine is a potential neuroprotective agent.

Keywords: Lidocaine, Anesthesia, Neuroprotection, Cadmium toxicity