Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

LncRNA TUG1 facilitates the development of endometrial cancer via interaction with FXR1

Zhongjie Guo^1,2 , Xuan Lin^1,2, Xiaoxia Guo²

¹The Affiliate Reproductive Hospital of Sichuan Genitalia Hygiene Research Center, Chengdu 610032; ²Department of Obstetrics and Gynecology, The 3rd Affiliated Hospital of Guangdong pharmaceutical University, Guangzhou 510410, China.

For correspondence:- Zhongjie Guo Email: Consult_dr_kwok@163.com Tel:+8615920364376

Accepted: 27 August 2021 Published: 30 September 2021

Citation: Guo Z, Lin X, Guo X. LncRNA TUG1 facilitates the development of endometrial cancer via interaction with FXR1. Trop J Pharm Res 2021; 20(9):1839-1844 doi: 10.4314/tjpr.v20i9.9

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the potential influence of long non-coding RNA (lncRNA) TUG1 on the development of endometrial cancer (EC).

Methods: A total of 24 paired EC species and paracancerous species were collected, and the differential expressions of TUG1 in them were determined. The regulatory effects of TUG1 on proliferative and migratory potential in Ishikawa and HEC-1A cells were assessed using cell counting kit-8 (CCK-8) and Transwell assay, respectively. Potential protein binding TUG1 was predicted by bioinformatics analysis and subsequently verified using RIP (RNA-Binding Protein Immunoprecipitation) assay. Rescue experiments were conducted to uncover the mechanism of TUG1 in regulating the development of EC.

Results: TUG1 was highly expressed in EC species and cell lines. Higher levels of TUG1 was observed in EC patients with metastases than those without metastatic cancer (p < 0.05). Overexpression of TUG1 markedly facilitated proliferative and migratory potential in EC cells. Taurine-upregulated gene 1 (TUG1) directly bound Fragile X-related protein 1 (FXR1) and positively regulated its level (p < 0.05). Through interaction with FXR1, TUG1 stimulated the malignant development of EC.

Conclusion: LncRNA TUG1 is upregulated in EC species, which facilitates proliferative and migratory potentials in EC cells by interacting with FXR1.

Keywords: Endometrial cancer, Taurine-upregulated gene 1, Fragile X-related protein 1, Proliferative and migratory potential