Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Mesenchymal stem cells promote incision wound repair in a mouse model

Dong-Jie Li, Chuan-An Shen, Tian-Jun Sun, Lin Zhang, Hu-Ping Deng, Jia-Ke Chai

Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing, 100048, China;

For correspondence:- Jia-Ke Chai Email: chaijiake403@gmail.com Tel:+861066867072

Received: 15 April 2016 Accepted: 9 May 2017 Published: 29 June 2017

Citation: Li D, Shen C, Sun T, Zhang L, Deng H, Chai J. Mesenchymal stem cells promote incision wound repair in a mouse model. Trop J Pharm Res 2017; 16(6):1317-1323 doi: 10.4314/tjpr.v16i6.15

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the wound healing process via the application of mesenchymal stem cells (MSCs) in a mouse model.

Methods: MSCs were collected from the bone marrow of the femur and tibia of 6 – 12-week-old C57BL/6 mice. Full-thickness cutaneous wounds (4 × 2 cm) were made by incision on the dorsal side of the mice. The wound was then subjected to one of four random treatments: phosphate-buffered saline (PBS) solution, 3T3 fibroblasts, naive MSCs, or interferon gamma-activated MSCs. Chalkley method was used to determine vascular density. A score was given, for each field examined, for CD31-positive areas, and the results of blind analysis were confirmed by independent analysis of a second evaluator.

Results: The tensile strength of the wound area was significantly lower in older versus younger mice (p ≤ 0.0007). Only one quarter of the mean force was required to disrupt wound integrity in older mice compared to young mice. Treatment with MSCs showed positive effects on wound healing. Activated MSCs showed the greatest efficacy at a dosage of 5 × 10⁴ activated MSCs/8 cm² of wound area or 6, 250 cells/cm².

Conclusions: The results suggest that MSC therapies enhance the tissue regeneration capacity in mice, especially in older populations, through effective transdifferentiation into the epithelium.

Keywords: Mesenchymal stem cell, wound healing, mouse