Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-1254 inhibits proliferation, migration and invasion of human brain tumour cell lines

Gang Li¹, Dong-Gang Zhao¹, Lai Jiang¹, Yu-Hua Guan¹, Hua Tang¹, Gang Zheng¹, Xin Huang¹, Yan Wang²

¹Department of Neurosurgery; ²Department of Gynaecology, The First People's Hospital of Yichang, China Three Gorges University, Yichang, Hubei 443000, China.

For correspondence:- Yan Wang Email: WyllieCckech@yahoo.com Tel:+867176222800

Accepted: 12 October 2017 Published: 31 January 2018

Citation: Li G, Zhao D, Jiang L, Guan Y, Tang H, Zheng G, et al. MiR-1254 inhibits proliferation, migration and invasion of human brain tumour cell lines. Trop J Pharm Res 2018; 17(1):35-40 doi: 10.4314/tjpr.v17i1.6

© 2018 The authors.
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Abstract

Purpose: To investigate the expression of miR-1254 in 5 astrocytoma cell lines, and the mechanism involved.

Methods: Total RNA was isolated by RNeasy RNA isolation kit while cDNA was prepared by RevertAid cDNA synthesis kit. The transcripts were analysed by real-time polymerase chain reaction (RT-PCR). Transfection of miR-1254 was carried out using FuGENE HD (Promega). Apoptosis was determined by DAPI, acridine orange (AO)/ethidium bromide (EB) and annexin V/PI double staining. Cell migration and invasion were investigated by wound healing and Martigel invasion assays, respectively. Protein expression was measured by western blotting.

Results: expression of miR-1254 was significantly down-regulated in the astrocytoma cell lines when compared to normal astrocyte cells (p < 0.05). Ectopic expression of miR-1254 in astrocytoma SW 1088 cells inhibited cell proliferation via initiation of apoptosis and cell cycle arrest. Over-expression of miR-1254 also led to significant decrease in cell migration and invasion of SW 1088 astrocytoma cells (p < 0.05).

Conclusion: The results show that the expression of miR-1254 is down-regulated in astrocytoma cell lines, but over-expression of miR-1254 inhibits proliferation of the cell lines via cell cycle arrest and apoptosis. Thus, miR-1254 has promising potential for use in the treatment of brain tumour

Keywords: Brain tumour, Astrocytoma, miR-1254, Apoptosis, Cell migration