Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-196a and miR-122 as potential markers in cholestatic liver disease induced by drugs

Zefeng Kang¹, Yongsheng Yang¹ , Yekai Lu², Qianqian Chen¹, Ran Han¹, Wei Lu¹, Mingfeng Zhang¹, Zhiyong Ma³, Guiqin Huang¹, Huixuan Xu¹

¹Department of Liver Diseases Branch, Cangzhou Infectious Disease Hospital, Cangzhou, Hebei 061001, China; ²Department of Infectious Disease, Cangzhou Infectious Disease Hospital, Cangzhou, Hebei 061001, China; ³Department of Clinical Laboratory, Cangzhou Infectious Disease Hospital, Cangzhou, Hebei 061001, China.

For correspondence:- Yongsheng Yang Email: shenjiqiao17022924@163.com

Accepted: 26 November 2021 Published: 31 December 2021

Citation: Kang Z, Yang Y, Lu Y, Chen Q, Han R, Lu W, et al. MiR-196a and miR-122 as potential markers in cholestatic liver disease induced by drugs. Trop J Pharm Res 2021; 20(12):2567-2571 doi: 10.4314/tjpr.v20i12.16

© 2021 The authors.
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Abstract

Purpose: To determine the role of miR-196a and miR-122 in cholestatic liver disease (CSLD) induced by drugs.

Methods: Sixty (60) children treated in Cangzhou Infectious Disease Hospital for drug-induced CSLD from January 2017 to January 2020 were recruited as the CSLD group, and 60 healthy people in the same period were enrolled as the control group. Polymerase chain reaction (PCR) was applied to evaluate the expression of serum miR-196a and miR-122, while enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of nuclear factor erythroid 2-related factor 2 (Nrf–2) and heme oxygenase-1 (HO-1). Diagnostic efficacy of miR-196a and miR-122 was assessed using receiver operating characteristic (ROC) curve.

Results: Compared to the control group, miR-196a and miR-122 in CSLD group were highly-expressed (p < 0.05). The serum expression of miR-196a in patients with mild, moderate and severe CSLD was noticeably higher than for control group (p < 0.05); the expression level of moderate and severe CSLD was lower than that of mild CSLD (p < 0.05). The serum miR-122 expression of patients with mild, moderate and severe CSLD was also higher compared to control group (p < 0.05). expressions of miR-196a and miR-122 were positively correlated with Nrf-2 and HO-1.

Conclusion: The expression of miR-196a and miR-122 in the serum of patients with drug-induced CSLD significantly increases, but decreases with the severity of CSLD. Thus, these molecules can potentially as biomarkers for the condition and prognosis of patients with CSLD.

Keywords: MiR-196a, MiR-122, Cholestatic liver disease, Diagnostic value