Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-208a reduces inflammatory responses in heart failure rats through β-catenin pathway

Yanrong Song¹, Yu Guo², Jie Qin¹, Xiaojing Jia³, Chentao Yang¹

¹Department of Laboratory, Xingtai Medical College, Xingtai, China; ²Department of CT and Magic Resonance Imaging, Xingtai Third Hospital/Xingtai Cardiovascular Hospital, Xingtai, China; ³Department of Internal Medicine, Xingtai Third Hospital/Xingtai Cardiovascular Hospital, Xingtai, China.

For correspondence:- Chentao Yang Email: xtyct1980@163.com Tel:+8615831919908

Accepted: 31 July 2023 Published: 31 August 2023

Citation: Song Y, Guo Y, Qin J, Jia X, Yang C. MiR-208a reduces inflammatory responses in heart failure rats through β-catenin pathway. Trop J Pharm Res 2023; 22(8):1567-1572 doi: 10.4314/tjpr.v22i8.6

© 2023 The authors.
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Abstract

Purpose: To investigate the effect of micro-ribonucleic acid (miR)-208a on heart failure (HF) in rats through β-catenin pathway.

Methods: A total of 24 specific pathogen-free female Sprague-Dawley rats were enrolled and randomly divided into 3 equal groups, namely, control (normal group), model, and study group (miR-208a), with 8 rats each. Echocardiography was utilized to evaluate cardiac function, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was applied to examine cardiomyocyte apoptosis. Finally, expression levels of interleukin (IL)-6 and IL-10 were determined using enzyme-linked immunosorbent assay (ELISA). expression of matrix metalloproteinases (MMPs) was determined via immunohistochemistry assay, while western blotting was used to measure expression of β-catenin.

Results: The mRNA expression level of miR-208a was significantly lower in model group than control and study group (p < 0.05). Cardiac function of rats in model group was significantly better than other groups (p < 0.05). Cardiomyocyte apoptosis was significantly increased in model group than in other groups (p < 0.05). Furthermore, expression levels of MMPs, IL-6 and IL-10 in model group were elevated in comparison with those in study and control groups (p < 0.05).

Conclusion: MiR-208a reduces inflammatory response and deposition of extracellular matrix in rats with HF through inhibition of β-catenin signaling pathway, thereby restoring cardiac function.

Keywords: MiR-208a, Inflammatory Response, Heart failure, β-Catenin pathway