Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-495-3p facilitates colon cancer cell proliferation via Wnt/^6;-catenin signaling pathway by restraining Wnt inhibitory factor

Lin Lin^1,2, Gangling Tong³, Meixiang Li², Aixue Liu², Senming Wang¹

¹Department of Oncology, Zhujiang Hospital, Southern Medical University, 253 Middle Industrial Avenue, Guangzhou, China; ²Department of Oncology, Shenzhen Second Peopleâ€™s Hospital; ³Department of Oncology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, PR China.

For correspondence:- Senming Wang Email: 13902404566@126.com

Accepted: 19 August 2017 Published: 30 September 2017

Citation: Lin L, Tong G, Li M, Liu A, Wang S. MiR-495-3p facilitates colon cancer cell proliferation via Wnt/^6;-catenin signaling pathway by restraining Wnt inhibitory factor. Trop J Pharm Res 2017; 16(9):2113-2120 doi: 10.4314/tjpr.v16i9.10

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To demonstrate whether miR-495-3p promote the occurrence of colon cancer and development of colon cancer stem cells by inhibiting Wnt inhibitory factor (WIF1).

Methods: The level of MiRNA and mRNA in cells were tested by real-time polymerase chain reaction (RT-PCR). Cell viability was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Cell spheroid formation was measured by colony assay. expression protein was tested using Western blotting. β-catenin binding ability was detected by chromatin immunoprecipitation (ChIP) assay. MiRNA target gene was defined by luciferase assay.

Results: Compared with normal colon cells and tissue, miR-495-3p is elevated in colon cancer cells and tissues, which regulate proliferation, level of stemness factors SOX-9, Bmil, and OCT-4 in HCT-116 cells, even spheroid formation. Overexpression of miR-495-3p inhibits the expression of WIF1 in HCT-116 cells and promotes colon tumorigenesis by binding with 3’-UTR. MiR-495-3p inhibitor downregulated WIF1-enhanced sphere formation of colon cancer cells.

Conclusion: These results indicate that miR-495-3p/WIF1 can modulate the development of colon cancer and is a potential target for prevention and treatment of cancer.

Keywords: MiR-495-3p, Wnt inhibitory factor, Colon cancer, Stemness, Tumorigenesis