Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-541-3p suppresses gastric cancer via negative regulation of HSF1

Zihan Zheng , Peng Zhou, Yangyang Xiao, Qian Liu, Tao Wan

Department of Gastrointestinal Surgery, Jiangxi Provincial People's Hospital, Nanchang City, Jiangxi Province 330006, China;

For correspondence:- Zihan Zheng Email: zzh041585@126.com Tel:+8679187721299

Accepted: 10 October 2021 Published: 30 November 2021

Citation: Zheng Z, Zhou P, Xiao Y, Liu Q, Wan T. MiR-541-3p suppresses gastric cancer via negative regulation of HSF1. Trop J Pharm Res 2021; 20(11):2293-2998 doi: 10.4314/tjpr.v20i11.9

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To explore the effects of miR-541-3P on the expression of heat shock transcription factor 1 (HSF1) in gastric cancer cells (GC).

Methods: The MicroRNA Target Prediction Database was used to predict whether miR-541-3p interacts with HSF1. Interaction was assessed by dual-luciferase reporter assays. Furthermore, miR-541-3p mRNA levels in GC cell lines were determined by qRT-PCR. Human GC cell lines MKN45 and NCI-N87 were transfected with miR-541-3p mimic. Cell apoptosis, proliferation, invasion, and migration were evaluated using flow cytometry, apoptosis assays, Edu assays, CCK-8 assays, and transwell assays, respectively. Caspase-3, Bcl-2, and cleaved caspase-3 expression levels were determined by western blot.

Results: expression of miR-541-3p was significantly down-regulated in GC cells. Functionally, miR-541-3p mimic inhibited GC cell proliferation, migration, and invasion and induced apoptosis in vitro (p < 0.01). Mechanistically, miR-541-3p interacted with HSF1 and inhibited its expression. Overexpression of HSF1 counteracted the effects of miR-541-3p mimic in GC cells.

Conclusion: These results indicate that miR-541-3p suppresses the development of GC by targeting HSF1 and thus, is a possible strategy for for the management of GC.

Keywords: MiR-541-3p, Gastric cancer, Cell proliferation, Cell migration, Cell invasion, Apoptosis, Heat shock transcription factor 1