Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Oroxylin A attenuates cytokines in neonatal asthmatic mice by regulating Notch signaling pathway

Ping Hua, Wei Wang, Lina Xiao, Hongjie Tao

Department of Pulmonary and Critical Care Mediline, Tonglu First People's Hospital, Tonglu, Hangzhou, Zhejiang province, China, 311500;

For correspondence:- Hongjie Tao Email: huaping1984@163.com Tel:+8657164399695

Accepted: 20 May 2021 Published: 30 June 2021

Citation: Hua P, Wang W, Xiao L, Tao H. Oroxylin A attenuates cytokines in neonatal asthmatic mice by regulating Notch signaling pathway. Trop J Pharm Res 2021; 20(6):1145-1150 doi: 10.4314/tjpr.v20i6.7

© 2021 The authors.
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Abstract

Purpose: To investigate the protective effect of oroxylin A against neonatal asthma in mice.

Methods: Asthma was induced in P12 mice with ovalbumin (OVA) immediately after litter (day 0), and on day 14 of protocol. Oroxylin A was given daily in two doses (2 and 10 mg/kg, i.p.) from day 0 to 14, 2 h before the administration of OVA. The protective effect of oroxylin A against asthma was determined by evaluating lung function and levels of cytokines and IgE in OVA-challenged mice. Moreover, Western blot assay and quantitative real-time polymerase chain reaction (qRT-PCR) were used to determine the effect of oroxylin A on Notch signaling pathway in OVA-challenged mice.

Results: Oroxylin A improved lung function in OVA-challenged mice. There were significant (p < 0.01) reductions in levels of IgE and cytokines in the BALF of oroxylin A-treated group, when compared to OVA group of mice. Furthermore, treatment with oroxylin A significantly ameliorated the OVA-induced changes in protein expressions of Notch1, Jagged1, NICD, PTEN, PI3K and Akt in the CD+ cells of OVA-challenged mice (p < 0.01).

Conclusion: These results indicate that oroxylin A improves lung function and reduces the level of cytokines in neonatal asthmatic rat by downregulating Notch signaling. Thus, oroxylin A may be clinically beneficial in the management of asthma.??????

Keywords: Asthma, Oroxylin A, Cytokine, Neonatal, Notch signal route