Mohammad Reza Mehrabi1,
Dariush Norouzian1,
Mohammad Ali Shokrgozar2,
Tayebeh Toliyat3,
Mohsen Chiani1,
Ali Farhangi1,
Fariba Alambin1,4,
Azim Akbarzadeh1 
For correspondence:- Azim Akbarzadeh Email: Azimakbarzadeh@pasteur.ac.ir Tel:+982166468763
Received: 30 August 2016 Accepted: 10 April 2017 Published: 29 May 2017
Citation: Mehrabi MR, Norouzian D, Shokrgozar MA, Toliyat T, Chiani M, Farhangi A, et al. Pegylated niosomal nanoparticles loaded with vincristine: Characterization and in vitro evaluation. Trop J Pharm Res 2017; 16(5):975-980 doi: 10.4314/tjpr.v16i5.2
© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the effect of pegylated niosomal vincristine (VCR) on enhanced performance, drug resistance and prolonged blood circulation time.
Methods: Pegylated niosomal VCR was synthesized by reverse phase evaporation. The mean diameter, size distribution, and zeta potential of pegylated niosomal VCR were evaluated using a Zetasizer. The half-maximal concentration (IC50) values of pegylated niosomal VCR and standard VCR were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The impact of pegylated niosomal VCR on apoptosis and cell cycle of BCL1 lymphoma cancer cells were investigated.
Results: The mean diameter, size distribution and zeta potential of pegylated niosomal VCR were 220 nm, 0.4, and –18.8 mV, respectively. Cell proliferation was evaluated using the MTT assay. The IC50 values of pegylated niosomal VCR and standard VCR were 1.6 and 3.5 μg/mL, respectively, after a 24-h incubation. The cytotoxicity of pegylated niosomal VCR was twice that of standard VCR. Furthermore, flow cytometric analysis of the cell cycle showed that pegylated niosomal VCR induced greater mitotic arrest than did standard VCR.
Conclusion: The findings demonstrate the effective antitumor activity of pegylated niosomal VCR compared with standard VCR
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