Yi-ru Chen 
,
Bao-sheng Li
For correspondence:- Yi-ru Chen Email: 2091859818@qq.com Tel:+8615852436218
Received: 31 August 2014 Accepted: 27 March 2015 Published: 26 May 2015
Citation: Chen Y, Li B. Phytochemical composition and antihyperplasic effect of Fructus hordei G in Rats. Trop J Pharm Res 2015; 14(5):789-794 doi: 10.4314/tjpr.v14i5.7
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the phytochemical profile and antihyperplasic effect of ethanol extract of Fructus hordei. G (FHE) on hyperplasia of mammary gland (HMG) in rats.
Methods: The Fructus hordei. G extract (FHE) was obtained by a rotary vacuum evaporator. The total flavonoids, total alkaloids and total phenolic compounds in FHE were assayed by aluminum chloride colorimetric method, acid-dye colorimetry and Folin-Ciocalteu method, respectively. HMG model rats were prepared for the experiments by injecting estradiol and progesterone. After oral administration with 150, 300 and 600 mg/kg of FHE for 30 days, breast nipple height, and serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), 5-hydroxytryptamine (5-HT), estradiol (E2), progesterone (P), prolactin (PRL), follicle stimulating hormone (FSH), luteinizing hormone (LH) and gonadotropin-releasing hormone (GnRH) levels of the rats were measured by ELISA kits. The pathological morphology of mammary gland tissues was examined by optical microscopy.
Results: The ethanol extract of FHE contained 1.88 ± 0.05 mg total flavonoids/g as quercetin equivalent (QE), 21.93 ± 0.06 mg hordenine equivalent (HE) of total alkaloids/g extract and 70.64 ± 0.11 mg total phenols/g gallic acid equivalent (GAE). The extract significantly diminished breast nipple height, MDA, 5-HT, E2, PRL, FSH and GnRH levels, and increased SOD, GSH-Px, P and LH levels (p < 0.05). FHE inhibited the alveolus, lobules and secretion of the mammary gland.
Conclusion: Fructus hordei extract has significant antihyperplasia effect on mice mammary gland by regulating serum sex hormones.
Archives
News Updates
Fulltext in PDF