Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Preparation and Evaluation of Glibenclamide-Loaded Biodegradable Nanoparticles

Amulyaratna Behera , Sunit Kumar Sahoo

University Department of Pharmaceutical Sciences, Utkal University, VaniVihar Bhubaneswar, Orissa, India;

For correspondence:- Amulyaratna Behera Email: amulyaslmp@rediffmail.com

Received: 23 July 2011 Accepted: 16 March 2012 Published: 15 June 2012

Citation: Behera A, Sahoo SK. Preparation and Evaluation of Glibenclamide-Loaded Biodegradable Nanoparticles. Trop J Pharm Res 2012; 11(3):345-350 doi: 10.4314/tjpr.v11i3.2

© 2012 The authors.
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Abstract

Purpose: To formulate and evaluate glibenclamide (GB)-loaded poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) for controlled release.

Methods: GB-loaded PLGA NPs were prepared by solvent evaporation technique using methanol/dichloromethane (2:1) and characterized by transmission electron microscopy (TEM), and differential scanning calorimetry (DSC). The effect of stirring speed (250, 1000, 1500 and 2500 rpm) and drug: polymer ratio (1:1, 1: 2, 1:3 and 2:1) on particle size, size distribution, zeta potential, drug loading, encapsulation efficiency and drug release was also studied.

Results: Stable NPs were successfully prepared without any incompatibility, as indicated by TEM and DSC studies, respectively. As polymer and drug concentrations, and stirring speed increased, particle size, drug loading and encapsulation efficiency also increased. Increase in polymer concentration sustained drug release but reverse was obtained as drug concentration increased.

Conclusion: Controlled release biodegradable glibenclamide NPs can be efficiently prepared by emulsification solvent evaporation method suitably modulating processing variables.

Keywords: Poly (lactic-co-glycolic) acid, Nanoparticles, Glibenclamide, Transmission electron microscopy, Differential scanning calorimetry