Xianwen Wan1,
Haixia Gong2
,
Hongxia Leng3,
Yulian Zhang3,
1Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, China;
2Department of Anesthesiology and Operative Medicine, Medical Center of Anesthesiology and Pain, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330052, China;
3The First Clinical Medical College, Nanchang University, Nanchang, Jiangxi Province 330031, China.
For correspondence:- Haixia Gong
Email: gonghaixia3092601@163.com Tel:+8613870891388
Accepted: 24 February 2024
Published: 31 March 2024
Citation:
Wan X, Gong H, Leng H, Zhang Y,
Protective effect of remimazolam on neuronal cells from OGD/R-induced damage: An in vitro study. Trop J Pharm Res 2024; 23(3):537-542
doi:
10.4314/tjpr.v23i3.7
© 2024 The authors.
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Abstract
Purpose: To investigate the effect of remimazolam (Re) on oxygen glucose deprivation/reoxygenation (OGD/R) model, and to elucidate the mechanism of action.
Methods: The neuron cells were treated with OGD/R. MTT assays were performed to confirm The effect of Re on cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, while flow cytometry (FCM) assay was conducted to determine the apoptosis of OGD/R cells. Immunostaining assay and enzyme-linked immunosorbent assay (ELISA) were used to evaluate the effects of Re on mitochondria damage, while immunoblot assay was used unravel its mechanism of action.
Results: Remimazolam (Re) significantly enhanced OGD/R-induced neuronal survival (p < 0.05). In addition, it significantly inhibited OGD/R-induced neuronal apoptosis, and OGD/R-induced neuron mitochondrial damage. Furthermore, it suppressed MAPK pathway in OGD/R-induced neuron, thus protecting the neuronal cells from OGD/R-induced damage (p < 0.05).
Conclusion: Remimazolam protects neuronal cells from OGD/R-induced damage by mediating MAPK pathway. Therefore, it is a potential drug for the management of Ischemia-reperfusion injury (IRI). However, in vivo studies are required to buttress this.
Keywords: Remimazolam, Oxygen glucose deprivation/reoxygenation (OGD/R), Apoptosis, Mitochondrial damage, MAPK pathway, Ischemia-reperfusion injury