Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Regulation of RhoA/ROCK1 signaling pathway by miR-26b in sepsis-induced acute lung injury

Yi Mao¹, Yi Gao², Zhiwei Zhao¹, Jinbo Zhang¹, Meiping Dong¹, Liexiang Cao¹

¹Emergency Center, The First People's Hospital of Wenling, Wenling, Zhejiang 317500, China; ²Department of Anesthesiology, The First People's Hospital of Wenling, Wenling, Zhejiang 317500, China.

For correspondence:- Liexiang Cao Email: aornrutai.p@pnu.ac.th Tel:+8613758672656

Accepted: 18 July 2022 Published: 28 August 2022

Citation: Mao Y, Gao Y, Zhao Z, Zhang J, Dong M, Cao L. Regulation of RhoA/ROCK1 signaling pathway by miR-26b in sepsis-induced acute lung injury. Trop J Pharm Res 2022; 21(8):1633-1638 doi: 10.4314/tjpr.v21i8.8

© 2022 The authors.
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Abstract

Purpose: To investigate the role of miR-26b in the regulation of RhoA/ ROCK1 signaling pathway in acute lung injury (ALI) caused by sepsis.

Methods: Thirty male rats were randomized into sham group (SG), cecal ligation and puncture (CLP) group (CG) and miR-26b mimic group (MG). Hematoxylin and eosin (H & E) staining assay was performed to determine the pathological characteristics of rat lung tissues in each group, while enzyme-linked immunosorbent assay (ELISA) was conducted to determine TNF-α and IL-1β levels. The miR-26b expression was evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR), while RhoA and Rock1 protein levels were assessed using western blotting.

Results: The CG had significant lung injury in comparison with the SG. There were significant elevation in TNF-α and IL-1β levels (p < 0.05). RhoA and ROCK1 levels in lung tissue were noticeably elevated in CG (p < 0.05). After treatment, lung injury in MG was reduced in contrast to CG. The MG showed statistically significant decrease (p < 0.05) in the levels of TNF-α and IL-1β, while the lung tissue mRNA expression and the RhoA and ROCK1 expression levels were significantly reduced in MG (p < 0.05).

Conclusion: The MiR-26b mimics plays an important role in the treatment of ALI induced by sepsis in rats by regulating RhoA/ROCK1 signaling pathway. Thus, the findings of this study provide a theoretical basis for clinical studies on the use of miR-26b in the therapy of sepsis.

Keywords: miR-26b, RhoA/ROCK1, Sepsis, Acute lung injury