Open Access


Read more
image01

Online Manuscript Submission


Read more
image01

Submitted Manuscript Trail


Read more
image01

Online Payment


Read more
image01

Online Subscription


Read more
image01

Email Alert



Read more
image01

Original Research Article | OPEN ACCESS

Studies on the expression and biological functions of ZIC5 in hepatocellular carcinoma

Pingpo Ming1, Weixing Wang2 , Chunyan Li3, Yongfa Zheng1, Wei Ge1

1Department of Oncology; 2Department of General Surgery; 3Department of Obstetrics, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province 430060, China.

For correspondence:-  Weixing Wang   Email: wxwang999@163.com   Tel:+862788041911

Accepted: 29 October 2020        Published: 30 November 2020

Citation: Ming P, Wang W, Li C, Zheng Y, Ge W. Studies on the expression and biological functions of ZIC5 in hepatocellular carcinoma. Trop J Pharm Res 2020; 19(11):2295-2302 doi: 10.4314/tjpr.v19i11.7

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the expression of zinc finger protein of the cerebellum 5 (ZIC5) and its biological functions in hepatocellular carcinoma (HCC).
Methods: Sixty-five patients undergoing HCC surgery were selected. expression of ZIC5 in HCC and para-carcinoma tissue was examined by quantitative real time-polymerase chain reaction (qRT-PCR) and western blotting. The relationship between ZIC5 expression and clinicopathological features, postoperative survival rate, and prognosis of liver cancer patients was analyzed by t-test, Kaplan-Meier method, and Cox regression analysis, respectively. The effects of ZIC5 silencing on Huh-7 cell proliferation, migration, invasion, and apoptosis were assessed using Cell Counting Kit-8 (CCK-8), wound healing assay, Transwell assay, and flow cytometry, respectively.
Results: ZIC5 expression in liver cancer tissue was significantly higher than in the para-carcinoma tissue and was significantly correlated with TNM stage and differentiation degree (p < 0.001). The overall survival rate of patients with high ZIC5 expression level was significantly lower than that of patients with low ZIC5 expression (p < 0.01). ZIC5 expression, TNM stage, and differentiation degree were independent prognostic factors. ZIC5 silencing significantly inhibited the proliferative, migratory, invasive, and anti-apoptotic capacity of Huh-7 cells (p < 0.01).
Conclusion: ZIC5 is highly expressed in HCC, and this can promote liver cancer cell proliferation, migration, and invasion.

Keywords: Hepatocellular carcinoma, Cerebellar zinc finger structure 5, Prognosis, Migration, Proliferation

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

Article Tools

Share this article with



Article status: Free
Fulltext in PDF
Similar articles in Google
Similar article in this Journal:

Archives

2024; 23: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10. 
2023; 22: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2022; 21: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2021; 20: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2020; 19: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2019; 18: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2018; 17: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2017; 16: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2016; 15: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2015; 14: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2014; 13: 
1,   2,   3,   4,   5,   6,   7,   8,   9,   10,   11,   12. 
2013; 12: 
1,   2,   3,   4,   5,   6. 
2012; 11: 
1,   2,   3,   4,   5,   6. 
2011; 10: 
1,   2,   3,   4,   5,   6. 
2010; 9: 
1,   2,   3,   4,   5,   6. 
2009; 8: 
1,   2,   3,   4,   5,   6. 
2008; 7: 
1,   2,   3,   4. 
2007; 6: 
1,   2,   3,   4. 
2006; 5: 
1,   2. 
2005; 4: 
1,   2. 
2004; 3: 
1. 
2003; 2: 
1,   2. 
2002; 1: 
1,   2. 

News Updates