Hyun Kang1,3,
Sushruta Koppula1,
Hoi- Ki Kim2,
Tae-Kyu Park1
1Department of Biotechnology , Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University, Chungju, 380-701;
2Fanipin Korea Co, Ltd, Ochang 208-211;
3KuGen Healthcare Institute, Chungju, 380-150, Republic of Korea.
For correspondence:- Tae-Kyu Park
Email: tkpark@kku.ac.kr Tel:+82438403870
Received: 18 April 2013
Accepted: 23 May 2013
Published: 12 June 2013
Citation:
Kang H, Koppula S, Kim H, Park T.
Suaeda japonica Makino Attenuates Lipopolysaccharide-Induced Neuro-Inflammatory Responses in BV-2 Microglia via NF-kappa B Signaling. Trop J Pharm Res 2013; 12(3):351-356
doi:
10.4314/tjpr.v12i3.12
© 2013 The authors.
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Abstract
Purpose: To evaluate in vitro anti-oxidant and anti-neuro-inflammatory activities of Suaeda japonica extract (SJE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells.
Methods: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay was used to study the antioxidant effects. 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay was used to study cell viabilities. LPS-stimulated BV- microglial cells were used to study the gene expression and production of inflammatory mediators determined by Western blot analysis.
Results: SJE significantly inhibited the DPPH generated free radicals, and suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) in a concentration-dependent manner. It decreased LPS-induced expression of some inflammatory mediators and pro-inflammatory cytokines (cyclooxygenase-2 and interleukin (IL)-6). This suppression of inflammatory mediators was nuclear factor kappa-B (NF-κB)-dependent.
Conclusion: Our findings imply that SJE may be a potential therapeutic agent in regulating microglia-mediated neuroinflammatory responses observed in several neurodegenerative diseases.
Keywords: Suaeda japonica, Antioxidant activity, Anti-inflammatory activity, Microglial Cells, iNOS, IL-6