Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Systemic effect of Angipars on regulation of wound healing is mediated by CXC in diabetes

Farangis Fatehi¹, Gholamhossein Hassanshahi², Seyed Ebrahim Hosseini³, Ahmad Shabani Zade⁴, Mohammad Mohsen Taghavi⁴

¹Physiology-Pharmacology Research Center; ²Molecular Medicine Research Center; ³Department of Biology, Fars Sciences and Research Branch, Islamic Azad University, Fars; ⁴Department of Anatomy, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

For correspondence:- Mohammad Taghavi Email:

Received: 19 January 2014 Revised: 16 November 2014 Published: 30 January 2015

Citation: Fatehi F, Hassanshahi G, Hosseini SE, Zade AS, Taghavi MM. Systemic effect of Angipars on regulation of wound healing is mediated by CXC in diabetes. Trop J Pharm Res 2015; 14(1):79-85 doi: 10.4314/tjpr.v14i1.12

© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To measure CXCL10 as angiostatic, and CXCL1, CXCL12 as angiogenic chemokines in the tissues of wounds of diabetics following treatment with insulin, angipars (a herbal Iranian drug) and a combination of angipars and insulin.

Methods: Forty eight male Wistar rats weighing 200 - 250 g were used. The induction of diabetes was carried out with 50 mg/kg of STZ (streptozotocin). Approximately, 56 days following the induction of diabetes, the rats were injured to establish wound lesion. They were divided into four main groups: non-diabetic control group (received only saline), diabetes group without treatment (received only saline), diabetes group which received insulin (reference) as treatment, and diabetes group which received both insulin and angipars. After 12 days of treatment, the animals were subjected to blood sampling from retro-orbital vein and CXC chemokines were analyzed by Western blotting.

Results: The results show that the concentration of CXC10 decreased from 95 pg/ml in the diabetic control group to 40 and 10 pg/ml in the insulin and combined angipars/insulin groups, respectively (p ≤ 0.05). However, CXCL12 concentration was not changed among the various groups compared to the control group. In diabetic control and angipars-insulin groups, CXCL1 level (pg/ml) was 98 and 50, respectively, thus indicating that expression of CXCL1 chemokine decreased significantly (p ≤ 0.05).

Conclusions: Angipars, due probably to its richness in some natural compounds such as coumarin and flavonoids (which are antioxidants), mediates chemokines expression and may be effective in the regulation of angiogenesis and inflammation via balancing of chemokines expression.

Keywords: Diabetes mellitus, Angipars, Insulin, Chemokine, Angiostatic, Angiogenic