Lixing Dai1,
Ruiqing Diao2,
Jinghua Zhang3,
Mengying Cao1,
Hongli Gao4,
Bibo Tang5
1Department of General Practice, Hubei Province Third People's Hospital Affiliated to Jianghan University, Wuhan 430033;
2Department of Neurology, Gucheng County Hospital of Hebei Province, Hengshui 253800, Hebei Province;
3Department of Encephalopathy, Tianjin Beichen District Hospital of Traditional Chinese Medicine, Tianjin 300400;
4Department of Medical, Hubei Minzu University, Enshi 445000;
5Department of Critical Care Medicine, Hubei Province Third People's Hospital Affiliated to Jianghan University, Wuhan 430033, Hubei Province, China.
For correspondence:- Bibo Tang
Email: tzkf64@163.com
Accepted: 26 April 2021
Published: 30 May 2021
Citation:
Dai L, Diao R, Zhang J, Cao M, Gao H, Tang B.
Tetramethyl pyrazine exerts anti-apoptotic and antioxidant effects in a mouse model of MPTP-induced Parkinson's disease via regulation of the expression of Bax, Bcl-2, Nrf2 and GCLC.. Trop J Pharm Res 2021; 20(5):893-898
doi:
10.4314/tjpr.v20i5.2
© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Abstract
Purpose: To investigate the effect of tetramethyl pyrazine (TMP) on MPTP)-mediated neuronal apoptosis and oxidative imbalance in mice, and the mechanism of action involved.
Methods: Forty-five mice were assigned evenly to blank control, MPTP and TMP groups. The protein concentrations of Bax, Bcl-2, cytochrome C (Cyt c), Nrf2, GCLC and cleaved caspase-3; and levels of glutathione (GSH) and thiobarbituric acid reactive products (TBARS) were evaluated and compared amongst the groups.
Results: Cyt c, Bax, and cleaved caspase-3 protein levels in TMP group were significantly lower than those in MPTP group, while Bcl-2 protein expression was higher in TMP group than in MPTP mice (p < 0.05). Furthermore, TBARS was lower in TMP group than in MPTP group, while GSH level increased, relative to MPTP mice. The levels of Nrf2 and GCLC were significantly higher in TMP group than in MPTP group (p < 0.05).
Conclusion: Tetramethyl pyrazine exerts anti-apoptotic and antioxidant effects on MPTP-mediated Parkinsonism via regulation of the expressions of Bax, Bcl-2, Nrf2 and glutamate-cysteine ligase catalytic subunit. Thus, TMP has potential for use in the treatment Parkinson’s disease
Keywords: Tetramethyl pyrazine, Bax, Bcl-2, Nrf2, glutamate-cysteine ligase catalytic subunit, MPTP, Parkinsonism