H Ahiboh1,3, A J Djaman2, F H Yapi2, A Edjeme-Aké1, M L Hauhouot-Attoungbré1, E D Yayo1, D Monnet1
1Département de Biochimie Biologie Moléculaire, UFR des Sciences Pharmaceutiques et Biologiques, Université de Cocody - Abidjan BP V34; 2Laboratoire de Pharmacodynamie Biochimique - UFR de Biosciences, Université de Cocody - Abidjan BP V34; 3Département de Biochimie Fondamentale et Clinique - Institut Pasteur de Côte d'Ivoire - 01 BP 490 Abidjan 01.For correspondence:- H Ahiboh Email: Tel:(+225) 07 22 41 11
Published: 21 June 2008
Citation: Ahiboh H, Djaman AJ, Yapi FH, Edjeme-Aké A, Hauhouot-Attoungbré ML, Yayo ED, et al. Uptake and kinetic properties of choline and ethanolamine in Plasmodium falciparum. Trop J Pharm Res 2008; 7(2):953-959 doi: 10.4314/tjpr.v7i2.4
© 2008 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: The asexual proliferation of Plasmodium, inside the erythrocyte, is accompanied by the synthesis of huge quantities of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn). These needful phospholipids for the cytoplasmic membrane of the merozoites are provided by the precursors choline and ethanolamine. PtdCho and PtdEtn are synthesized by the parasite because the erythrocyte is unable to do it. In order to assess the dynamism of the phospholipid pathways, we aimed to investigate the respective shape of the uptake of choline and ethanolamine by Plasmodium falciparum.
Method: Time-course experiments and kinetic assays were performed respectively with fixed and ranged concentrations of radioactively-labelled choline and ethanolamine. The labelled-precursors were added in the culture of P. falciparum infected-erythrocytes and the incorporated molecules in phospholipids were measured with a scintigraph counter.
Result: The results showed that the incorporation of precursors in the infected-erythrocyte occurred with a Michaelis-Menten’s kinetic shape. According to the maximum rate (Vmax), the pathway of ethanolamine incorporation was faster than that of choline. Similarly, affinity for ethanolamine was greater than that of choline.
Conclusion: Although PtdCho is the major phospholipid in the membrane, this study rules out that the influx of ethanolamine in the infected-erythrocyte, in vivo conditions, is more dynamic than choline.
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