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Original Research Article | OPEN ACCESS

Vasodilating effect of Hypericum revolutum (Vahl) (Clusiaceae) methanol extract in rats

Noha Z Timraz1, Hany M El-Bassossy2, Sabrin RM Ibrahim3,4, Ali M El-Halawany5, Ibrahim A Shehata5, Omar S Aljohani1 Hossam M Abdallah1,5

1Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 2Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Zagazig 44519; 3Batterjee Medical College, PO Box 6231, North Obhur, Prince Abdullah Al-Faisal Street, 21442 Jeddah, Saudi Arabia; 4Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526; 5Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.

For correspondence:-  Hossam Abdallah   Email: hmafifi@kau.edu.sa   Tel:966544733110

Accepted: 22 April 2021        Published: 31 May 2021

Citation: Timraz NZ, El-Bassossy HM, Ibrahim SR, El-Halawany AM, Shehata IA, Aljohani OS, et al. Vasodilating effect of Hypericum revolutum (Vahl) (Clusiaceae) methanol extract in rats. Trop J Pharm Res 2021; 20(5):1003-1007 doi: 10.4314/tjpr.v20i5.17

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the vasodilating effect of Hypericum revolutum Vahl (Clusiaceae) in rats.
Methods: H. revolutum aerial parts were extracted with methanol. The total methanol extract was fractionated with chloroform to yield fraction I. The remaining aqueous solution was chromatographed on Diaion HP-20 using water, 50 % methanol, and methanol to yield three fractions (II- IV). Total methanol extract and fractions I-IV were applied to phenylephrine-pre-contracted (10 μM) rat aortic rings at doses of 1, 3, and 10 μg/mL. Subsequent decreases in aortic tension were recorded by an isometric force transducer to evaluate the vasodilation. Column chromatography was utilized to separate the active components of the bioactive fraction. 
Results: Remarkable decreases in aortic tension (p<0.05) revealed that fraction I (3 and 10 μg/mL) produced a vasodilating effect, whereas fractions III and IV did not possess any substantial effect. Vasodilation induced by fraction I was endothelial-dependent because it was significantly (p<0.05) blocked by endothelial denudation. Phytochemical inspection of fraction I led to the isolation of β-sitosterol (1), 1,7-dihydroxyxanthone (euxanthone) (2), and 2,3,4-tirmethoxy xanthone (3).
Conclusion: Fraction I of the H. revolutum extract was responsible for its vasodilating effect. This fraction may be used as a possible anti-hypertensive preparation after in vivo testing and successful clinical trials.

Keywords: Vasodilation, Hypericum revolutum, Clusiaceae, Nitric oxide

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)

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