Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

lncRNA ENST00000434223 exerts regulatory effects on NSCLC cells via Wnt/β-catenin signaling pathway

Jinge Shen¹, Song Yu², Wenmei Zhang³, Danrong Yang³

¹Department of Emergency, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Jiaotong University Affiliated Sixth Peopleâ€™s Hospital, No. 600 Yi Shan Road, Shanghai 200233, China; ²Department of General Surgery, Shanghai Jiaotong University Affiliated Sixth Peopleâ€™s Hospital, No. 600 Yi Shan Road, Shanghai 200233, China; ³Department of Respiratory and Critical Care Medicine, Shanghai Jiaotong University Affiliated Sixth Peopleâ€™s Hospital, No. 600 Yi Shan Road, Shanghai 200233, China.

For correspondence:- Danrong Yang Email: yangdanrong17@163.com Tel:+8618930172711

Accepted: 30 March 2023 Published: 29 April 2023

Citation: Shen J, Yu S, Zhang W, Yang D. lncRNA ENST00000434223 exerts regulatory effects on NSCLC cells via Wnt/β-catenin signaling pathway. Trop J Pharm Res 2023; 22(4):731-739 doi: 10.4314/tjpr.v22i4.4

© 2023 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To identify the role and underlying mechanism of lncRNA ENST00000434223 (lncRNA ENST) in the occurrence of NSCLC.

Methods: Quantitative real time-polymerase chain reaction (qRT-PCR) was used to evaluate relative lncRNA ENST, β-catenin, Wnt1, and cyclin D1 expression levels in NSCLC tissues, cells, and sera. Proliferation of NSCLC cells was assessed using CCK-8 assay, while Transwell assay was utilized in evaluating changes in cell invasion/migration. Changes in Wnt1, cyclin D1, and β-catenin expressions were determined using qRT-PCR, while alterations in their protein expressions were determined by western blot (WB). In vivo experiments were conducted in nude mice to investigate the effects of lncRNA ENST on tumor growth.

Results: lncRNA ENST expression was low in sera, cells, and tissues of NSCLC (p <0.05). Tumor metastasis and size, lymphatic metastasis, and clinical stage were correlated with lncRNA ENST expression (p < 0.05). It was found that ENST could be applied as independent prognostic factor in NSCLC patients. Upregulated expression of lncRNA ENST restrained cell migration, proliferation, and invasion (p < 0.05). Compared with t NC group, lncRNA ENST expression decreased expressions of Wnt1, cyclin D1, and β-catenin in SPC-A1 cells and A549 cells (p < 0.05).

Conclusion: lncRNA ENST inhibits the proliferation and apoptosis of NSCLC cells via Wnt/β-catenin pathway. Thus, it is a possible biomarker for NSCLC, and provides a novel insight into the clinical management of NSCLC.

Keywords: lncRNA ENST, Cell invasion, Cell migration, Wnt/β-catenin, NSCLC, Cell proliferation