Chao Zhao1,
Xiong Lu1,
Yunjin Deng1,
Yifan Huang1,2 
,
Bin Liu1,2 ,
For correspondence:- Yifan Huang Email: zjhyfang@163.com Tel:+8659183530197
Received: 10 November 2014 Accepted: 13 March 2015 Published: 26 April 2015
Citation: Zhao C, Lu X, Deng Y, Huang Y, Liu B, Purification and characterization of thermostable cellulase from Consortium XM70 in terrestrial hot spring with sugarcane bagasse. Trop J Pharm Res 2015; 14(4):591-598 doi: 10.4314/tjpr.v14i4.5
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To better understand the sugarcane bagasse (SCB) degradation process and obtain thermostable cellulase from terrestrial hot spring.
Methods: Molecular community structure of the newly selected thermophilic bacterial consortium XM70 was determined using the method of the full-length 16S rRNA library-based analysis. The thermostable CMCase was purified with ion-exchange and gel filtration chromatography.
Results: Sequence-based identification of species belonging to the genera, Geobacillus, Desulfotomaculum, Bacillus, Exiguobacterium, Paenibacillus and Enterobacter were identified. The maximal activities of carboxymethyl cellulase (CMCase), filter paper cellulase and β-glucosidase of the consortium XM70 were obtained after incubation at 60 °C and pH 6.0 (4 days), 80 °C and pH 7.0 (2 days) and 70 °C and pH 8.0 (4 days), respectively. The yield of reducing sugars in the culture broth achieved 0.11 g.g-1 dry SCB. An extracellular CMCase from consortium XM70 (XM70-CMCase) was purified 7.9-fold to apparent homogeneity with a recovery of 65.41 % and its molecular mass was about 31.0 kDa. Maximum CMCase activity of the purified XM70-CMCase was 3.77 U/mg at 70 °C and pH 7.0. CMCase activity maintained about its maximal value of 70 % after incubation at 80 °C for 60 min.
Conclusion: Due to its high temperature stability, the purified XM70-CMCase may be useful for industrial application such as biofuel, animal feed industry, paper industry and clarification of fruit juices.
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