Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antioxidant and Antihaemolytic Activities of the Leaves of Kefe cumin (Laser trilobum L) Umbelliferae

Mohammad Ali Ebrahimzadeh¹, Seyed Mohammad Nabavi^1,2, Seyed Fazel Nabavi¹, Bahman Eslami³, Zahra Rahmani⁴

¹Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari; ²Young Researchers Club, Islamic Azad University, Ghaemshahr Branch, Ghaemshahr; ³Department of Biology, Islamic Azad University, Ghaemshahr branch; ⁴Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

For correspondence:- Zahra Rahmani Email: zadeh20@yahoo.com Tel:+981513543081

Received: 9 January 2010 Accepted: 23 July 2010 Published: 17 October 2010

Citation: Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Eslami B, Rahmani Z. Antioxidant and Antihaemolytic Activities of the Leaves of Kefe cumin (Laser trilobum L) Umbelliferae. Trop J Pharm Res 2010; 9(5):441-449 doi: 10.4314/tjpr.v9i5.3

© 2010 The authors.
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Abstract

Purpose: To investigate the antioxidant and antihaemolytic properties of the leaves of Laser trilubum grown in Gaduk, Iran.

Methods: The antioxidant and antihaemolytic activities of the hydroalcohol extract of L. trilobum L. leaf were investigated by haemoglobin-induced linoleic acid peroxidation, scavenging of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, nitric oxide and hydrogen peroxide, as well as by assessment of reducing power and Fe²⁺chelating activity, using standard methods. Phenol and flavonoid contents were determined as gallic acid and quercetin equivalents, respectively.

Results: The extract showed antioxidant activity in some models, with 50 % inhibitory concentration (IC₅₀) for DPPH radical-scavenging activity being 147.1 ± 7.5 mg ml^-1. The extract showed good nitric oxide-scavenging activity of between 0.1 and 1.6 mg ml^-1 (IC₅₀ = 517.7 ± 23.1 vs. 20 ± 0.01 µg ml^-1 for quercetin), weak Fe²⁺ chelating ability (IC₅₀, 906.9 ± 37.8 mg ml^-1), and low antioxidant activity in haemoglobin-induced linoleic acid system. However, it was capable of scavenging hydrogen peroxide in a concentration-dependent manner while also exhibiting potent antihaemolytic activity against H₂O₂ - induced haemolysis (IC₅₀, 169.6 ± 6.9 mg ml^-1). Total phenolic content was 75 ± 3 mg gallic acid equivalent/g and total flavonoid content 59.2 ± 2.1 mg quercetin equivalent/g. The amounts of gallic acid, quercetin and rutin were 0.99 ± 0.04, 0.63 ± 0.01 and < 0.10 μg/mg, respectively.

Conclusion: L. trilobum exhibited good but varying levels of antioxidant and antihaemolytic activities in nearly all the models studied, when compared with controls.

Keywords: Antioxidant activity, Laser trilobum, Antihaemolytic, Flavonoids, Kefe cumin