Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Development of a New RP-UPLC Method for the Determination of Rabeprazole Sodium in Pharmaceutical Formulation and Application in Dissolution Studies

K Karunakaran¹ , G Navaneethan¹, K P Elango²

¹Department of Chemistry, Sona College of Technology, Anna University, Salem 636 005; ²Department of Chemistry, Gandhigram Rural Institute, Deemed University, Gandhigram 624302, India.

For correspondence:- K Karunakaran Email: drkk@sonatech.ac.in Tel:+914274099998

Received: 1 March 2011 Accepted: 20 August 2011 Published: 23 October 2011

Citation: Karunakaran K, Navaneethan G, Elango KP. Development of a New RP-UPLC Method for the Determination of Rabeprazole Sodium in Pharmaceutical Formulation and Application in Dissolution Studies. Trop J Pharm Res 2011; 10(5):655-661 doi: 10.4314/tjpr.v10i5.15

© 2011 The authors.
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Abstract

Purpose: To develop a reverse phase ultra-performance liquid chromatographic (RP-UPLC) method for the estimation of rabeprazole sodium in tablet formulations.

Methods: Chromatographic separation was achieved on a Waters Acquity BEH C₁₈ (50 x 2.1 mm, particle size 1.7 µm) column using an isocratic method with mobile phase composed of acetonitrile and phosphate buffer (pH 7.4) in the ratio of 35:65 (v/v). The flow rate was 0.4 ml/min, temperature of the column was maintained at ambient, injection volume was 5 µL and detection was made at 280 nm. The run time was as short as 2 min. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity.

Results: The developed method was linear for rabeprazole sodium from 0.03 - 30 µg/ml and the linear regression obtained was > 0.999. Precision, evaluated by intra- and inter-day assay,s had relative standard deviation (R.S.D) values within 1.5 %. Recovery data were in the range 98.0 - 101.5 % with R.S.D. values < 1.5 %.

Conclusion: The method is precise, accurate, linear, robust and fast. The short retention time of 1.49 min allows the analysis of a large number of samples in a short period of time and, therefore, should be cost-effective for routine analysis in the pharmaceutical industry.

Keywords: Ultra performance liquid chromatography, Assay, Rabeprazole sodium, Validation