Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

A Protease Isolated from the Latex of Plumeria rubra Linn (Apocynaceae) 2: Anti-inflammatory and Wound-Healing Activities

Indranil Chanda¹ , Usha Sarma², Sanat K Basu³, Mangala Lahkar⁴, Sadhan K Dutta⁵

¹Girijananda Chowdhury Institute of Pharmaceutical Science, Guwahati, Assam-781017; ²Department of Pathology, Gauhati Medical College and Hospital, Guwahati, Assam-781032; ³Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal-700032; ⁴Department of Pharmacology, Gauhati Medical College and Hospital, Guwahati, Assam-781032; ⁵A College of Pharmacy, Bengal School of Technology, Hooghly, West Bengal- 712102, India.

For correspondence:- Indranil Chanda Email: ichanda@sify.com Tel:+919957179226

Received: 12 May 2011 Accepted: 15 October, 2011 Published: 25 December 2011

Citation: Chanda I, Sarma U, Basu SK, Lahkar M, Dutta SK. A Protease Isolated from the Latex of Plumeria rubra Linn (Apocynaceae) 2: Anti-inflammatory and Wound-Healing Activities. Trop J Pharm Res 2011; 10(6):755-760 doi: 10.4314/tjpr.v10i6.8

© 2011 The authors.
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Abstract

Purpose: The aim of this study was to investigate the anti-inflammatory and wound-healing activities of the protease isolated from the latex of Plumeria rubra Linn.

Methods: The protease was isolated from the latex of the plant by acetone precipitation method and given a trivial name, Plumerin-R. The anti-inflammatory activity of the protease was based on its effects on carrageenan-induced paw oedema in rats. Its wound healing effect was investigated using an excision wound rat model.

Results: Four hours after treatment, the reduction in carrageenan-induced rat paw oedema by 20, 40 and 80 mg/kg body weight of plumerin-R was 21.6, 33.8 and 48.8 %, respectively, while oedema reduction by indomethacin (10 mg/kg) was 58.0 %. Plumerin-R showed significant (p < 0.05) wound closure and epithelialization time compared with control.

Conclusion: This study demonstrates that the protease, Plumerin-R, has significant anti-inflammatory and wound-healing properties.

Keywords: Protease, Plumerin-R, Anti-inflammatory, Excision wound, Healing