Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Development of Ultraviolet Spectrophotometric Method for Analysis of Lornoxicam in Solid Dosage Forms

Sunit Kumar Sahoo¹ , Ranjit Kumar Giri¹, Sachin V Patil², Amulya Ratna Behera¹, Ranjit Mohapatra¹

¹University Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar, Orissa; ²Department of Pharmaceutics, Shree Santkrupa College of Pharmacy, Ghogaon, Tal. Karad, Dist. Satara, Maharashtra, India.

For correspondence:- Sunit Sahoo Email: sahoosunitkumar@rediffmail.com

Received: 21 January 2011 Accepted: 20 February 2012 Published: 24 April 2012

Citation: Sahoo SK, Giri RK, Patil SV, Behera AR, Mohapatra R. Development of Ultraviolet Spectrophotometric Method for Analysis of Lornoxicam in Solid Dosage Forms. Trop J Pharm Res 2012; 11(2):269-273 doi: 10.4314/tjpr.v11i2.14

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: An ultraviolet spectrophotometric system was developed and validated for the quantitative determination of lornoxicam in solid dosage forms.

Methods: Lornoxicam was dissolved in 0.01M NaOH and analysed using ultraviolet (UV) spectrophotometry. Various analytical parameters such as linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization (ICH) guidelines.

Results: Absorbance maximum in 0.01M NaOH was 377 nm. Beer’s law was obeyed over the concentration range of 2 - 20 mg/ml with a correlation coefficient (r²) value of 0.999. Percent range of error was 0.344 and 0.261 at 0.05 and. 0.01 confidence limits, respectively. Intra- and inter-day precision (% RSD) at different concentration levels were < 2 %, indicating that the proposed derivative spectrophotometric method is highly reproducible during one run and between different runs; LOD and LOQ were 0.105 and 0.318 mg/ml, respectively signifying that it can be adopted for routine quality testing. Mean recovery was 100.82 % for tablets. Low values of % RSD indicate the reliability of the proposed method.

Conclusion: The proposed method is highly sensitive, precise, accurate, cost-effective, reliable and rapid for the estimation of lornoxicam in solid dosage forms.

Keywords: Lornoxicam, UV Spectrophotometry, Quantitative determination, Solid dosage forms