Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Cisplatin Induces Up-Regulation of KAI1, a Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell Line

Mohammad Javad Mokhtari¹ , Azim Akbarzadeh², Mehrdad Hashemi¹, Gholamreza Javadi¹, Reza Mahdian³, Soheil Ghasemi², Ahmad Reza Kamiab¹, Zhilla Hoseineian¹, Mohsen Chiani², Mahdieh Sadat Taghavi¹

¹Department of Biology, Science and Research Branch, Islamic Azad University; ²Pilot Biotechnology Department; ³Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

For correspondence:- Mohammad Mokhtari Email: mjmokhtari@srbiau.ac.ir Tel:+982144865154

Received: 14 October 2011 Accepted: 6 June 2012 Published: 16 August 2012

Citation: Mokhtari MJ, Akbarzadeh A, Hashemi M, Javadi G, Mahdian R, Ghasemi S, et al. Cisplatin Induces Up-Regulation of KAI1, a Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell Line. Trop J Pharm Res 2012; 11(4):523-529 doi: 10.4314/tjpr.v11i4.1

© 2012 The authors.
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Abstract

Purpose: To investigate the effect of cisplatin on cell toxicity and metastasis through modulation of KAI1 gene expression.

Methods: MCF-7cells were incubated with different concentrations of cisplatin for 24 h. RNA was extracted by trizol and cDNA synthesized. KAI1 and TBP were chosen as target and internal control genes, respectively. Specific primers were designed by primer express software, v.3.0. KAI1/TBP and gene expression ratio was calculated using the formula, 2 ^-W10;W10;Ct.

Results: Cisplatin exerted a dose-dependent inhibitory effect on the viability of highly metastatic MCF-7 cells. KAI1/TBP gene expression ratios were 1.97 ± 0.19 (p < 0.05), 2.96 ± 0.55 (p < 0.05), 9.06 ± 0.27 (p < 0.001) and 12.38 ± 0.88 (p < 0.01) in 10, 20, 50 and 100 μM concentrations of cisplatin.

Conclusion: These findings indicate that cisplatin can inhibit metastasis by up-regulating KAI1 gene in MCF-7cells.

Keywords: Cisplatin; KAI1; Metastasis; Breast Cancer; Real-time PCR