Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Hypolipidemic, Hypoglycemic and Anti-oxidant Activities of Flower Extracts of Allamanda Violacea A. DC (Apocynaceae)

Arun Sethi¹ , Rohit Prakash¹, Akriti Bhatia¹, Gitika Bhatia², Ashok K Khanna², Swayam Prakash Srivastava²

¹Department of Chemistry, University of Lucknow, Lucknow-226007; ²Division of Biochemistry, Central Drug Research Institute, Lucknow, India.

For correspondence:- Arun Sethi Email: alkaarunsethi@rediffmail.com Tel:+919415396239

Received: 3 July 2011 Accepted: 3 May 2012 Published: 16 August 2012

Citation: Sethi A, Prakash R, Bhatia A, Bhatia G, Khanna AK, Srivastava SP. Hypolipidemic, Hypoglycemic and Anti-oxidant Activities of Flower Extracts of Allamanda Violacea A. DC (Apocynaceae). Trop J Pharm Res 2012; 11(4):595-604 doi: 10.4314/tjpr.v11i4.10

© 2012 The authors.
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Abstract

Purpose: To investigate the anti-dyslipidemic, anti-oxidant and anti-diabetic activities of the aqueous extract and solvent fractions of A. violacea flowers.

Methods: The aqueous extract was fractionated into petroleum ether, ether, chloroform, chloroform-methanol (4:1) and chloroform-methanol (3:2) fractions. Lipid lowering activity was evaluated in two models, viz, triton WR-1339 - induced hyperlipimea in rats as well as fructose-rich high fat diet. To assess anti-oxidant activity, in-vitro model of non-enzymic superoxide hydroxyl radicals and microsomal lipid peroxidation by non-enzymic inducer was adopted. Hypoglycemic activity was evaluated by sucrose-loaded rat model.

Results: Amongst the fractions, ether and chloroform fractions caused marked decrease in the levels of total cholesterol (Tc), triglycerides (Tg), plasma lipids (Pl), and protein by 24, 23, 23 and 22 %, and 24, 22, 23 and 19 %, respectively. In rats fed with high fat diet (HFD), ether and chloroform fractions lowered Tc, Tg and, Pl by 26, 25 and 26 %, and 18, 19 and 20 %, respectively. Significant decrease in superoxide anions, hydroxyl radicals and microsomal lipid peroxidation by ether and chloroform fractions was also observed. Chloroform, chloroform-methanol (4:1) and chloroform-methanol (3:2) fractions showed antihyperglycaemic activity to the extent of 25.2, 21.6 and 23.2 %, respectively.

Conclusion: The flowers of this plant, especially the ether and chloroform extracts, may be suitable as an anti-oxidant supplement for lipid management.

Keywords: Allamanda violacea flowers, Anti-hyperlipidemic, Anti-hyperglycemic, Anti-oxidant